PMID- 16458457
OWN - NLM
STAT- MEDLINE
DCOM- 20070816
LR  - 20060529
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 115
IP  - 1-3
DP  - 2006 Jun 15
TI  - High risk of false positive results in a widely used diagnostic test for 
      detection of the porcine reproductive and respiratory syndrome virus (PRRSV).
PG  - 21-31
AB  - During 2003 and 2004, increasing numbers of positive PRRSV RT-PCR results were 
      reported from herds negative for PRRSV infection. Interestingly, three herds 
      represent nucleus herds with no animal contacts from outside and without clinical 
      symptoms of PRRS until now. Since these positive results that were obtained using 
      a PCR protocol adapted to routine laboratory conditions could not be reproduced 
      with other PRRSV specific RT-PCRs, controlled negative and positive samples were 
      used to examine this phenomenon. A RT-PCR assay for detection and differential 
      diagnosis of the European and North American genotypes of the porcine 
      reproductive and respiratory syndrome virus (PRRSV) according to the method 
      previously published by Oleksiewicz et al. [Oleksiewicz, M.B., Botner, A., 
      Madsen, K.G., Storgaard, T., 1998. Sensitive detection and typing of porcine 
      reproductive and respiratory syndrome virus by RT-PCR amplification of whole 
      viral genes. Vet. Microbiol. 64, 7-22] was investigated in parallel to another 
      recently published method [Pesch, S., 2003. Etablierung einer Nachweismethode fur 
      die zwei Genotypen von dem porcine reproductive and respiratory syndrome virus 
      (PRRSV) und ein Beitrag zu seiner molekularen Epidemiologie. Thesis, Institute of 
      Virology, Faculty of Veterinary Medicine, University of Leipzig]. A panel of 228 
      clinical samples sent in for PRRSV routine diagnostics served as test panel. It 
      was found that both methods have similar analytical sensitivity. However, the 
      primers published by Oleksiewicz were shown to yield a very high proportion of 
      false positive results under routine diagnostic laboratory conditions, i.e. they 
      resulted in RT-PCR products with non-PRRSV sequences, that were indistinguishable 
      from truly positive reagents in standard gel electrophoresis settings. The reason 
      for and possible implications of this finding as well as the risk of modifying 
      published methods without control are discussed.
FAU - Fetzer, C
AU  - Fetzer C
AD  - BioScreen European Veterinary Disease Management Center GmbH, Mendelstr. 11, 
      48149 Munster, Germany.
FAU - Pesch, S
AU  - Pesch S
FAU - Ohlinger, V F
AU  - Ohlinger VF
LA  - eng
PT  - Comment
PT  - Journal Article
DEP - 20060203
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
CON - Vet Microbiol. 1998 Nov;64(1):7-22. PMID: 9874099
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - DNA, Viral/analysis/chemistry
MH  - Diagnosis, Differential
MH  - False Positive Reactions
MH  - Gene Amplification
MH  - Humans
MH  - Molecular Sequence Data
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - RNA, Viral/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction/standards/*veterinary
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 2006/02/07 09:00
MHDA- 2007/08/19 09:00
CRDT- 2006/02/07 09:00
PHST- 2005/10/13 00:00 [received]
PHST- 2005/12/20 00:00 [revised]
PHST- 2006/01/03 00:00 [accepted]
PHST- 2006/02/07 09:00 [pubmed]
PHST- 2007/08/19 09:00 [medline]
PHST- 2006/02/07 09:00 [entrez]
AID - S0378-1135(06)00004-6 [pii]
AID - 10.1016/j.vetmic.2006.01.001 [doi]
PST - ppublish
SO  - Vet Microbiol. 2006 Jun 15;115(1-3):21-31. doi: 10.1016/j.vetmic.2006.01.001. 
      Epub 2006 Feb 3.