PMID- 16458588
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20200929
IS  - 1386-6346 (Print)
IS  - 1386-6346 (Linking)
VI  - 34
IP  - 3
DP  - 2006 Mar
TI  - Reduced expression and functional impairment of Toll-like receptor 2 on dendritic 
      cells in chronic hepatitis C virus infection.
PG  - 156-62
AB  - BACKGROUND: Dendritic cells (DCs) utilize Toll-like receptors (TLRs) to sense 
      virus and initiate immune responses. We aimed at elucidating the roles of TLRs on 
      DCs in hepatitis C virus (HCV) infection. METHODS: Monocyte-derived DCs were 
      obtained from 32 healthy volunteers (HV) and 30 chronically HCV-infected patients 
      (CH). TLR2, TLR3 and TLR4 expressions on immature DCs were quantified by 
      real-time quantitative RT-PCR. We stimulated DCs with specific TLR ligands and 
      examined DC maturation, cytokine production and ability to stimulate allogeneic 
      CD4(+) T cells. RESULTS: TLR2 expression on immature DCs was lower in the CH 
      group, whereas those of TLR3 or TLR4 were not different between the groups. Each 
      TLR ligand induced DC maturation and stimulated them to release comparable levels 
      of IL-12p70, IL-6, IL-10, TNF-alpha and IFN-beta between the groups. TLR2 and 
      TLR4 ligands enhanced DC ability to stimulate T cell proliferation, with the 
      degree due to the TLR2 ligand being lower in the CH group. CONCLUSIONS: In HCV 
      infection, the TLR2 expression on DCs is reduced and TLR2-stimulated DCs show 
      lesser ability to proliferate T cells than healthy counterparts, suggesting that 
      the TLR2 system is involved in HCV-induced immunopathogenesis.
FAU - Yakushijin, Takayuki
AU  - Yakushijin T
AD  - Department of Gastroenterology and Hepatology, Osaka University Graduate School 
      of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
FAU - Kanto, Tatsuya
AU  - Kanto T
FAU - Inoue, Michiyo
AU  - Inoue M
FAU - Oze, Tsugiko
AU  - Oze T
FAU - Miyazaki, Masanori
AU  - Miyazaki M
FAU - Itose, Ichiyo
AU  - Itose I
FAU - Miyatake, Hideki
AU  - Miyatake H
FAU - Sakakibara, Mitsuru
AU  - Sakakibara M
FAU - Kuzushita, Noriyoshi
AU  - Kuzushita N
FAU - Hiramatsu, Naoki
AU  - Hiramatsu N
FAU - Takehara, Tetsuo
AU  - Takehara T
FAU - Kasahara, Akinori
AU  - Kasahara A
FAU - Hayashi, Norio
AU  - Hayashi N
LA  - eng
PT  - Journal Article
DEP - 20060203
PL  - Netherlands
TA  - Hepatol Res
JT  - Hepatology research : the official journal of the Japan Society of Hepatology
JID - 9711801
EDAT- 2006/02/07 09:00
MHDA- 2006/02/07 09:01
CRDT- 2006/02/07 09:00
PHST- 2005/11/27 00:00 [received]
PHST- 2005/12/28 00:00 [revised]
PHST- 2005/12/28 00:00 [accepted]
PHST- 2006/02/07 09:00 [pubmed]
PHST- 2006/02/07 09:01 [medline]
PHST- 2006/02/07 09:00 [entrez]
AID - S1386-6346(05)00477-8 [pii]
AID - 10.1016/j.hepres.2005.12.010 [doi]
PST - ppublish
SO  - Hepatol Res. 2006 Mar;34(3):156-62. doi: 10.1016/j.hepres.2005.12.010. Epub 2006 
      Feb 3.