PMID- 16458913
OWN - NLM
STAT- MEDLINE
DCOM- 20070726
LR  - 20171116
IS  - 0021-9797 (Print)
IS  - 0021-9797 (Linking)
VI  - 298
IP  - 2
DP  - 2006 Jun 15
TI  - Study on the formation and depolymerization of acridine orange dimer in acridine 
      orange-sodium dodecyl benzene sulfonate-protein system.
PG  - 757-64
AB  - Experiment indicates that the fluorescence of acridine orange (AO) can be greatly 
      quenched by anionic surfactant sodium dodecyl benzene sulfonate (SDBS), but when 
      protein is added into the AO-SDBS system, the fluorescence intensity of the 
      latter is enhanced. It is considered that SDBS can promote the formation of AO 
      dimer, resulting in the quenching of the fluorescence of AO. When bovine serum 
      albumin (BSA) is added into AO-SDBS system, BSA and SDBS can interact and form 
      negative micelle-like cluster complex with "aromatic ring stacking," which 
      destroys the formation conditions of AO dimer and makes some AO dimers turn into 
      monomer, resulting in the fluorescence enhancement of AO-SDBS system. Whereas the 
      positive AO and residual AO dimer are dissolved in the negative BSA-SDBS cluster 
      through electrostatic and hydrophobic forces and form a large association. Here, 
      the fluorescence enhancement of AO-SDBS is considered to originate from the 
      hydrophobic microenvironment provided by BSA and SDBS, the depolymerization of AO 
      dimer and intermolecular energy transfer between BSA and AO.
FAU - Wang, Fei
AU  - Wang F
AD  - Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry 
      of Education, School of Chemistry and Chemical Engineering, Shandong University, 
      Jinan 250100, PR China.
FAU - Yang, Jinghe
AU  - Yang J
FAU - Wu, Xia
AU  - Wu X
FAU - Wang, Xiaobo
AU  - Wang X
FAU - Feng, Lishun
AU  - Feng L
FAU - Jia, Zhen
AU  - Jia Z
FAU - Guo, Changying
AU  - Guo C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060203
PL  - United States
TA  - J Colloid Interface Sci
JT  - Journal of colloid and interface science
JID - 0043125
RN  - 0 (Benzenesulfonates)
RN  - 0 (Polymers)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 60NSK897G9 (dodecylbenzenesulfonic acid)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/chemistry/*metabolism
MH  - Animals
MH  - Benzenesulfonates/chemistry/*metabolism
MH  - Cattle
MH  - Dimerization
MH  - Energy Transfer
MH  - Polymers/*chemistry
MH  - Protein Binding
MH  - Serum Albumin, Bovine/chemistry/*metabolism
MH  - Spectrometry, Fluorescence
EDAT- 2006/02/07 09:00
MHDA- 2007/07/27 09:00
CRDT- 2006/02/07 09:00
PHST- 2005/11/17 00:00 [received]
PHST- 2006/01/11 00:00 [revised]
PHST- 2006/01/11 00:00 [accepted]
PHST- 2006/02/07 09:00 [pubmed]
PHST- 2007/07/27 09:00 [medline]
PHST- 2006/02/07 09:00 [entrez]
AID - S0021-9797(06)00034-8 [pii]
AID - 10.1016/j.jcis.2006.01.028 [doi]
PST - ppublish
SO  - J Colloid Interface Sci. 2006 Jun 15;298(2):757-64. doi: 
      10.1016/j.jcis.2006.01.028. Epub 2006 Feb 3.