PMID- 16458961
OWN - NLM
STAT- MEDLINE
DCOM- 20060530
LR  - 20220318
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 27
IP  - 16
DP  - 2006 Jun
TI  - Electrospun silk-BMP-2 scaffolds for bone tissue engineering.
PG  - 3115-24
AB  - Silk fibroin fiber scaffolds containing bone morphogenetic protein 2 (BMP-2) 
      and/or nanoparticles of hydroxyapatite (nHAP) prepared via electrospinning were 
      used for in vitro bone formation from human bone marrow-derived mesenchymal stem 
      cells (hMSCs). BMP-2 survived the aqueous-based electrospinnig process in 
      bioactive form. hMSCs were cultured for up to 31 days under static conditions in 
      osteogenic media on the scaffolds (silk/PEO/BMP-2, silk/PEO/nHAP, 
      silk/PEO/nHAP/BMP-2) and controls (silk/PEO, silk/PEO extracted). Electrospun 
      silk fibroin-based scaffolds supported hMSC growth and differentiation toward 
      osteogenic outcomes. The scaffolds with the co-processed BMP-2 supported higher 
      calcium deposition and enhanced transcript levels of bone-specific markers than 
      in the controls, indicating that these nanofibrous electrospun silk scaffolds 
      were an efficient delivery system for BMP-2. X-ray diffraction (XRD) analysis 
      revealed that the apatite formed on the silk fibroin/BMP-2 scaffolds had higher 
      crystallinity than on the silk fibroin scaffold controls. In addition, nHAP 
      particles were incorporated into the electrospun fibrous scaffolds during 
      processing and improved bone formation. The coexistence of BMP-2 and nHAP in the 
      electrospun silk fibroin fibers resulted in the highest calcium deposition and 
      upregulation of BMP-2 transcript levels when compared with the other systems. The 
      results suggest that electrospun silk-fibroin-based scaffolds are potential 
      candidates for bone tissue engineering. Furthermore, the mild aqueous process 
      required to spin the fibers offers an important option for delivery of labile 
      cytokines and other components into the system.
FAU - Li, Chunmei
AU  - Li C
AD  - Bioengineering and Biotechnology Center, Department of Biomedical Engineering, 
      Tufts University, Medford, MA 02155, USA.
FAU - Vepari, Charu
AU  - Vepari C
FAU - Jin, Hyoung-Joon
AU  - Jin HJ
FAU - Kim, Hyeon Joo
AU  - Kim HJ
FAU - Kaplan, David L
AU  - Kaplan DL
LA  - eng
GR  - EB002520/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060203
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Collagen Type I)
RN  - 0 (IBSP protein, human)
RN  - 0 (Insect Proteins)
RN  - 0 (Integrin-Binding Sialoprotein)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (Silk)
RN  - 0 (Transforming Growth Factor beta)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9007-49-2 (DNA)
RN  - 9007-76-5 (Fibroins)
RN  - 91D9GV0Z28 (Durapatite)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Birefringence
MH  - Bombyx
MH  - Bone Morphogenetic Protein 2
MH  - Bone Morphogenetic Proteins/chemistry/*pharmacology
MH  - Calcification, Physiologic/drug effects
MH  - Calcium/analysis
MH  - Cell Culture Techniques/methods
MH  - Collagen Type I/genetics
MH  - DNA/analysis
MH  - Durapatite/analysis/chemistry
MH  - Extracellular Matrix/ultrastructure
MH  - Fibroins/chemistry
MH  - Gene Expression/drug effects/genetics
MH  - Humans
MH  - Insect Proteins/chemistry/pharmacology
MH  - Integrin-Binding Sialoprotein
MH  - Male
MH  - Mesenchymal Stem Cells/chemistry/metabolism/ultrastructure
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Nanostructures/chemistry/ultrastructure
MH  - Osteogenesis/*drug effects
MH  - Polyethylene Glycols/chemistry
MH  - Sialoglycoproteins/genetics
MH  - Silk/*chemistry
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Tissue Engineering/*methods
MH  - Transforming Growth Factor beta/chemistry/*pharmacology
MH  - X-Ray Diffraction
EDAT- 2006/02/07 09:00
MHDA- 2006/05/31 09:00
CRDT- 2006/02/07 09:00
PHST- 2005/09/13 00:00 [received]
PHST- 2006/01/09 00:00 [accepted]
PHST- 2006/02/07 09:00 [pubmed]
PHST- 2006/05/31 09:00 [medline]
PHST- 2006/02/07 09:00 [entrez]
AID - S0142-9612(06)00021-4 [pii]
AID - 10.1016/j.biomaterials.2006.01.022 [doi]
PST - ppublish
SO  - Biomaterials. 2006 Jun;27(16):3115-24. doi: 10.1016/j.biomaterials.2006.01.022. 
      Epub 2006 Feb 3.