PMID- 16459653
OWN - NLM
STAT- MEDLINE
DCOM- 20070302
LR  - 20060207
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 32
IP  - 12
DP  - 2005 Dec
TI  - Construction and characterization of a recombinant pseudorabies virus expressing 
      porcine reproductive and respiratory syndrome virus GP5.
PG  - 1248-55
AB  - The GP5 gene of porcine reproductive and respiratory syndrome virus (PRRSV) was 
      integrated into the TK gene locus of pseudorabies virus (PRV) vaccine strain 
      Bartha-K61, resulting in a TK- and gE- negative recombinant PRV harboring GP5 
      gene, designated as rPRV-GP5. The in vitro expression of the GP5 by 
      rPRV-GP5-infected cells was analyzed by single-step growth analysis,Western 
      blot,and indirect immunofluorescence test. It was shown that GP5 gene can be 
      expressed authentically in the cytoplasm of rPRV-GP5-infected cells. Compared to 
      its parental virus, rPRV-GP5 showed no obvious difference regarding viral 
      replication and cytopathogenic effects in several cell cultures. Four 
      PRV-negative sheep immunized intramuscularly with 10(6.0) PFU of rPRV-GP5 were 
      fully protected from challenge with 10(3) LD50 of highly virulent PRV S strain of 
      porcine origin. Ten PRV- and PRRSV-negative piglets given intranasally with 
      10(7.0) PFU of rPRV-GP5 and challenged intranasally with 10(5.0) TCID50 of 
      virulent PRRSV CH-1a strain at day 63 post-inoculation developed antibodies 
      against PRRSV 3, 5, 14 days post-challenge, as revealed by indirect 
      immunofluorescence test, enzyme-linked immunosorbent assay and virus 
      neutralization test. The results suggest that rPRV-GP5 is capable of inducing 
      anamnestic immune response to PRRS in inoculated animals.
FAU - Tian, Zhi-Jun
AU  - Tian ZJ
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, CAAS, Harbin 150001, China.
FAU - Qiu, Hua-Ji
AU  - Qiu HJ
FAU - Ni, Jian-Qiang
AU  - Ni JQ
FAU - Zhou, Yan-Jun
AU  - Zhou YJ
FAU - Cai, Xue-Hui
AU  - Cai XH
FAU - Zhou, Guo-Hui
AU  - Zhou GH
FAU - Wang, Yun-Feng
AU  - Wang YF
FAU - Tong, Guang-Zhi
AU  - Tong GZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
SB  - IM
MH  - Animals
MH  - Genetic Vectors
MH  - Herpesvirus 1, Suid/*genetics
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - Pseudorabies
MH  - *Virus Replication
EDAT- 2006/02/08 09:00
MHDA- 2007/03/03 09:00
CRDT- 2006/02/08 09:00
PHST- 2006/02/08 09:00 [pubmed]
PHST- 2007/03/03 09:00 [medline]
PHST- 2006/02/08 09:00 [entrez]
PST - ppublish
SO  - Yi Chuan Xue Bao. 2005 Dec;32(12):1248-55.