PMID- 16460913
OWN - NLM
STAT- MEDLINE
DCOM- 20060629
LR  - 20071114
IS  - 0890-8508 (Print)
IS  - 0890-8508 (Linking)
VI  - 20
IP  - 2
DP  - 2006 Apr
TI  - Dendrimer FISH detection of single-copy intervals in acute promyelocytic 
      leukemia.
PG  - 114-20
AB  - Acute promyelocytic leukemia (AML-M3) is characterized by a translocation between 
      chromosomes 15 and 17 [t(15;17)]. The detection of t(15;17) at the single cell 
      level, is commonly done by fluorescence in situ hybridization (FISH) using 
      recombinant locus specific genomic probes greater than 14 kilobases kb in length. 
      To allow a more thorough study of t(15;17), we designed small (0.9-3.6 kb), 
      target-specific, single-copy probes from the human genome sequence. A novel 
      detection approach was evaluated using moieties possessing more fluorophores, DNA 
      dendrimers (up to 375 fluorophores per dendrimer). Two detection approaches were 
      evaluated using the dendrimers: (1) dendrimers modified with anti-biotin 
      antibodies for detection of biotinylated bound probes, and (2) dendrimers 
      modified with 45-base long oligonucleotides designed from the single-copy probes, 
      for direct detection of the target region. The selectivity of the probes was 
      confirmed via indirect labeling with biotin/digoxigenin by nick translation, with 
      detection efficiencies between 50 and 90%. Furthermore, the scFISH probes were 
      successfully detected on metaphase cells with anti-biotin dendrimer conjugates 
      and on interphase cells with 45-base modified dendrimers. Our results bring up 
      the possibility to detect target regions of less than 1 kb, which will be a great 
      contribution to high-resolution analysis of genomic sequences.
FAU - Mora, Johanna R
AU  - Mora JR
AD  - Department of Chemistry, University of Kansas, Lawrence, 66045, USA.
FAU - Knoll, Joan H M
AU  - Knoll JH
FAU - Rogan, Peter K
AU  - Rogan PK
FAU - Getts, Robert C
AU  - Getts RC
FAU - Wilson, George S
AU  - Wilson GS
LA  - eng
GR  - GM08359/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060207
PL  - England
TA  - Mol Cell Probes
JT  - Molecular and cellular probes
JID - 8709751
RN  - 0 (DNA Probes)
RN  - 0 (Dendrimers)
SB  - IM
MH  - Cell Line, Tumor
MH  - Chromosomes, Human, Pair 15/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - DNA Probes/chemistry
MH  - Dendrimers/chemistry
MH  - *Genome, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Leukemia, Promyelocytic, Acute/*genetics/pathology
MH  - Metaphase
MH  - *Translocation, Genetic
EDAT- 2006/02/08 09:00
MHDA- 2006/06/30 09:00
CRDT- 2006/02/08 09:00
PHST- 2005/07/23 00:00 [received]
PHST- 2005/11/09 00:00 [accepted]
PHST- 2006/02/08 09:00 [pubmed]
PHST- 2006/06/30 09:00 [medline]
PHST- 2006/02/08 09:00 [entrez]
AID - S0890-8508(05)00097-6 [pii]
AID - 10.1016/j.mcp.2005.11.005 [doi]
PST - ppublish
SO  - Mol Cell Probes. 2006 Apr;20(2):114-20. doi: 10.1016/j.mcp.2005.11.005. Epub 2006 
      Feb 7.