PMID- 16461583
OWN - NLM
STAT- MEDLINE
DCOM- 20060526
LR  - 20231105
IS  - 1040-4651 (Print)
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Linking)
VI  - 18
IP  - 3
DP  - 2006 Mar
TI  - High-resolution single-copy gene fluorescence in situ hybridization and its use 
      in the construction of a cytogenetic map of maize chromosome 9.
PG  - 529-44
AB  - High-resolution cytogenetic maps provide important biological information on 
      genome organization and function, as they correlate genetic distance with 
      cytological structures, and are an invaluable complement to physical sequence 
      data. The most direct way to generate a cytogenetic map is to localize 
      genetically mapped genes onto chromosomes by fluorescence in situ hybridization 
      (FISH). Detection of single-copy genes on plant chromosomes has been difficult. 
      In this study, we developed a squash FISH procedure allowing successful detection 
      of single-copy genes on maize (Zea mays) pachytene chromosomes. Using this 
      method, the shortest probe that can be detected is 3.1 kb, and two sequences 
      separated by approximately 100 kb can be resolved. To show the robust nature of 
      this protocol, we localized nine genetically mapped single-copy genes on 
      chromosome 9 in one FISH experiment. Integration of existing information from 
      genetic maps and the BAC contig-based physical map with the cytological structure 
      of chromosome 9 provides a comprehensive cross-referenced cytogenetic map and 
      shows the dramatic reduction of recombination in the pericentromeric 
      heterochromatic region. To establish a feasible mapping system for maize, we also 
      developed a probe cocktail for unambiguous identification of the 10 maize 
      pachytene chromosomes. These results provide a starting point toward constructing 
      a high-resolution integrated cytogenetic map of maize.
FAU - Wang, Chung-Ju Rachel
AU  - Wang CJ
AD  - Department of Molecular and Cell Biology, University of California, Berkeley, 
      California 94720, USA.
FAU - Harper, Lisa
AU  - Harper L
FAU - Cande, W Zacheus
AU  - Cande WZ
LA  - eng
SI  - GENBANK/AF375999
SI  - GENBANK/AY083961
SI  - GENBANK/AY321491
SI  - GENBANK/DQ351339
SI  - GENBANK/DQ352544
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20060203
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
SB  - IM
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Plant/*genetics
MH  - Cucurbita/genetics
MH  - Genes, Plant
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Molecular Sequence Data
MH  - Multigene Family/genetics
MH  - Pachytene Stage/genetics
MH  - Zea mays/*genetics
PMC - PMC1383631
EDAT- 2006/02/08 09:00
MHDA- 2006/05/27 09:00
PMCR- 2007/03/01
CRDT- 2006/02/08 09:00
PHST- 2006/02/08 09:00 [pubmed]
PHST- 2006/05/27 09:00 [medline]
PHST- 2006/02/08 09:00 [entrez]
PHST- 2007/03/01 00:00 [pmc-release]
AID - tpc.105.037838 [pii]
AID - 037838 [pii]
AID - 10.1105/tpc.105.037838 [doi]
PST - ppublish
SO  - Plant Cell. 2006 Mar;18(3):529-44. doi: 10.1105/tpc.105.037838. Epub 2006 Feb 3.