PMID- 16461718
OWN - NLM
STAT- MEDLINE
DCOM- 20060420
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 72
IP  - 2
DP  - 2006 Feb
TI  - Dynamic transition of a methanogenic population in response to the concentration 
      of volatile fatty acids in a thermophilic anaerobic digester.
PG  - 1623-30
AB  - In this study, the microbial community succession in a thermophilic methanogenic 
      bioreactor under deteriorative and stable conditions that were induced by 
      acidification and neutralization, respectively, was investigated using 
      PCR-mediated single-strand conformation polymorphism (SSCP) based on the 16S rRNA 
      gene, quantitative PCR, and fluorescence in situ hybridization (FISH). The SSCP 
      analysis indicated that the archaeal community structure was closely correlated 
      with the volatile fatty acid (VFA) concentration, while the bacterial population 
      was impacted by pH. The archaeal community consisted mainly of two species of 
      hydrogenotrophic methanogen (i.e., a Methanoculleus sp. and a Methanothermobacter 
      sp.) and one species of aceticlastic methanogen (i.e., a Methanosarcina sp.). The 
      quantitative PCR of the 16S rRNA gene from each methanogen revealed that the 
      Methanoculleus sp. predominated among the methanogens during operation under 
      stable conditions in the absence of VFAs. Accumulation of VFAs induced a dynamic 
      transition of hydrogenotrophic methanogens, and in particular, a drastic change 
      (i.e., an approximately 10,000-fold increase) in the amount of the 16S rRNA gene 
      from the Methanothermobacter sp. The predominance of the one species of 
      hydrogenotrophic methanogen was replaced by that of the other in response to the 
      VFA concentration, suggesting that the dissolved hydrogen concentration played a 
      decisive role in the predominance. The hydrogenotrophic methanogens existed close 
      to bacteria in aggregates, and a transition of the associated bacteria was also 
      observed by FISH analyses. The degradation of acetate accumulated during 
      operation under deteriorative conditions was concomitant with the selective 
      proliferation of the Methanosarcina sp., indicating effective acetate degradation 
      by the aceticlastic methanogen. The simple methanogenic population in the 
      thermophilic anaerobic digester significantly responded to the environmental 
      conditions, especially to the concentration of VFAs.
FAU - Hori, Tomoyuki
AU  - Hori T
AD  - Department of Biotechnology, Graduate School of Agricultural and Life Sciences, 
      The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Haruta, Shin
AU  - Haruta S
FAU - Ueno, Yoshiyuki
AU  - Ueno Y
FAU - Ishii, Masaharu
AU  - Ishii M
FAU - Igarashi, Yasuo
AU  - Igarashi Y
LA  - eng
SI  - GENBANK/AB200367
SI  - GENBANK/AB200368
SI  - GENBANK/AB200369
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Fatty Acids)
RN  - 0 (RNA, Archaeal)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Anaerobiosis
MH  - Base Sequence
MH  - Bioreactors/*microbiology
MH  - DNA, Archaeal/genetics
MH  - DNA, Bacterial/genetics
MH  - Fatty Acids/*metabolism
MH  - Genes, Archaeal
MH  - In Situ Hybridization, Fluorescence
MH  - Methane/biosynthesis
MH  - Methanobacteriaceae/genetics/*metabolism
MH  - Methanomicrobiaceae/genetics/*metabolism
MH  - Methanosarcina/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single-Stranded Conformational
MH  - RNA, Archaeal/genetics
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Waste Disposal, Fluid
PMC - PMC1392901
EDAT- 2006/02/08 09:00
MHDA- 2006/04/21 09:00
PMCR- 2006/06/01
CRDT- 2006/02/08 09:00
PHST- 2006/02/08 09:00 [pubmed]
PHST- 2006/04/21 09:00 [medline]
PHST- 2006/02/08 09:00 [entrez]
PHST- 2006/06/01 00:00 [pmc-release]
AID - 72/2/1623 [pii]
AID - 2053-05 [pii]
AID - 10.1128/AEM.72.2.1623-1630.2006 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2006 Feb;72(2):1623-30. doi: 
      10.1128/AEM.72.2.1623-1630.2006.