PMID- 16465242
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20060302
LR  - 20060208
IS  - 1350-9047 (Print)
IS  - 1350-9047 (Linking)
VI  - 4
IP  - 4
DP  - 1997 May
TI  - Expression of a defective mouse mammary tumor virus envelope glycoprotein 
      precursor which binds stably to GRP78 within the lumen of the endoplasmic 
      reticulum is associated with decreased glucocorticoid-induced apoptosis in mouse 
      lymphoma cells.
PG  - 283-8
AB  - Viral proteins inhibit apoptosis in host cells by a variety of mechanisms. This 
      report proposes an additional mechanism, based on the interaction of a mutant 
      mouse mammary tumor virus (MMTV) envelope glycoprotein precursor, Pr74, with the 
      stress protein GRP78 (BiP) within the lumen of the endoplasmic reticulum (ER) (J. 
      Biol. Chem. 268 7482-7488, 1993). We show that WEHI7.2 (W7.2) mouse lymphoma 
      cells, which do not express Pr74, are more sensitive to cell death induction by 
      the glucocorticoid hormone dexamethasone (dex), than W7MG1 cells, which were 
      derived by infecting W7.2 cells with MMTV and therefore express Pr74 under 
      control of the glucocorticoid-inducible MMTV promoter. Moreover, W7-ENV/N cells, 
      derived by stably transfecting W7.2 cells with a constitutively expressed cDNA 
      encoding mutant Pr74, were less sensitive to dex-induced cell death than control 
      transfectant W7-ENV/- cells. Among multiple W7-ENV/N subclones, susceptibility to 
      dex-induced cell death was inversely related to the level of Pr74 synthesis. The 
      interaction of Pr74 with GRP78 induces an increase in GRP78 synthesis. Thus, the 
      repression of cell death associated with Pr74 expression may be secondary to 
      elevated synthesis of GRP78, a stress protein previously implicated in protection 
      against cell death.
FAU - Lam, M
AU  - Lam M
AD  - Department of Medicine, Case Western Reserve University, Cleveland, Ohio 
      44106-4937, USA.
FAU - Stallcup, M
AU  - Stallcup M
FAU - Distelhorst, C W
AU  - Distelhorst CW
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cell Death Differ
JT  - Cell death and differentiation
JID - 9437445
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1999/11/10 00:00 [received]
PHST- 1999/01/09 00:00 [revised]
PHST- 1999/01/11 00:00 [accepted]
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - 4400249 [pii]
AID - 10.1038/sj.cdd.4400249 [doi]
PST - ppublish
SO  - Cell Death Differ. 1997 May;4(4):283-8. doi: 10.1038/sj.cdd.4400249.