PMID- 16465372
OWN - NLM
STAT- MEDLINE
DCOM- 20060328
LR  - 20190606
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 28
IP  - 3
DP  - 2006 Mar
TI  - Allelic imbalance at 11q23-q24 chromosome associated with estrogen and 
      radiation-induced breast cancer progression.
PG  - 667-74
AB  - Multiple genetic alterations are common in cancers including those of the breast. 
      The mechanisms leading to these alterations such as point mutations, gene 
      amplifications, deletions and replication error are often associated with 
      frequent and consistent loss of heterozygosity (LOH) or microsatellite 
      instability (MSI). Several cytological and molecular studies have shown high 
      frequency loss of genetic information on the long arm of chromosome 11 (i.e., 
      11q) in various primary breast cancers. In the present study allelic alterations 
      in a refined position on the long arm of chromosome 11 were studied to identify 
      the spectrum of induced damage at different stages of malignant transformation of 
      MCF-10F cell lines after exposure to high-LET radiation using alpha-particles and 
      exposure to estradiol by using PCR-single strand conformation polymorphism (SSCP) 
      and fluorescence in situ hybridization (FISH) analysis. Microsatellite markers 
      were selected from chromosome 11 (11q23-q24 loci) and it was found that frequency 
      of allelic imbalance occurs at different stages of tumor progression with a range 
      of 15-45% depending on the marker studied. These results strongly suggested the 
      presence of several tumor suppressor genes in this critical region of chromosome 
      11 (11q23-q24). It also represents the first indication of allele loss at these 
      loci in human breast epithelial cells induced by radiation and estrogen treatment 
      suggesting a potential interventional target in breast carcinogenesis.
FAU - Roy, D
AU  - Roy D
AD  - Center for Radiological Research, College of Physicians and Surgeons, Columbia 
      University, New York, NY 11973, USA. droy@bnl.gov
FAU - Calaf, G M
AU  - Calaf GM
FAU - Hande, M P
AU  - Hande MP
FAU - Hei, T K
AU  - Hei TK
LA  - eng
GR  - CA49062/CA/NCI NIH HHS/United States
GR  - ES05786/ES/NIEHS NIH HHS/United States
GR  - P30 ES09089/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Estrogens)
SB  - IM
MH  - Alpha Particles
MH  - Breast Neoplasms/genetics/pathology
MH  - Cell Line, Transformed
MH  - Cell Line, Tumor
MH  - Cell Transformation, Neoplastic/drug effects/*genetics/radiation effects
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Estrogens/*pharmacology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Loss of Heterozygosity
MH  - Microsatellite Repeats/genetics
EDAT- 2006/02/09 09:00
MHDA- 2006/03/29 09:00
CRDT- 2006/02/09 09:00
PHST- 2006/02/09 09:00 [pubmed]
PHST- 2006/03/29 09:00 [medline]
PHST- 2006/02/09 09:00 [entrez]
AID - 10.3892/ijo.28.3.667 [doi]
PST - ppublish
SO  - Int J Oncol. 2006 Mar;28(3):667-74. doi: 10.3892/ijo.28.3.667.