PMID- 16466382
OWN - NLM
STAT- MEDLINE
DCOM- 20060426
LR  - 20191210
IS  - 0168-6496 (Print)
IS  - 0168-6496 (Linking)
VI  - 55
IP  - 3
DP  - 2006 Mar
TI  - Detection of activity among uncultured Actinobacteria in a drinking water 
      reservoir.
PG  - 432-8
AB  - The abundance, identity and activity of uncultured Bacteria and Actinobacteria 
      present in a drinking water reservoir (North Pine Dam, Brisbane, Australia) were 
      determined using a combination of fluorescence in situ hybridization (FISH) alone 
      or with catalysed reporter deposition (CARD-FISH) with microautoradiography. The 
      CARD-FISH technique was modified relative to previous described procedures and 
      performed directly on gelatine cover slips in order to allow simultaneous 
      combination with microautoradiography. Almost twofold higher numbers of 
      microorganisms could be identified as either Bacteria or Actinobacteria using the 
      CARD-FISH technique as compared with the traditional FISH technique. A 
      combination of FISH or CARD-FISH with microautoradiography showed generally 
      higher activity among the Actinobacteria than among all Bacteria. Another 
      important observation was that many cells within the FISH-negative populations of 
      both Actinobacteria and Bacteria were actively assimilating thymidine. Thus, 
      great care should be taken when extrapolating the active fraction of a 
      prokaryotic community to be equivalent to the FISH-detectable population in such 
      environments. Bacterial groups within Actinobacteria produce the odours geosmin 
      and 2-methylisoborneol, which lower the quality of surface water when used for 
      drinking. The results indicate that combined microautoradiography and CARD-FISH 
      may serve as an effective tool when studying identity and activity of 
      microorganisms within freshwater environments.
FAU - Nielsen, Jeppe L
AU  - Nielsen JL
AD  - Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg 
      University, Aalborg, Denmark. jeppe@bio.aau.dk
FAU - Klausen, Cecilie
AU  - Klausen C
FAU - Nielsen, Per H
AU  - Nielsen PH
FAU - Burford, Michele
AU  - Burford M
FAU - Jorgensen, Niels O G
AU  - Jorgensen NO
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (Culture Media)
RN  - 0 (Naphthols)
RN  - 0 (Oligonucleotide Probes)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
RN  - MYW912WXJ4 (geosmin)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Actinobacteria/genetics/growth & development/*metabolism
MH  - Autoradiography
MH  - Bacteria/genetics/growth & development/metabolism
MH  - Catalysis
MH  - Colony Count, Microbial
MH  - Culture Media
MH  - Fresh Water/*microbiology
MH  - *Horseradish Peroxidase
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Naphthols
MH  - *Oligonucleotide Probes
MH  - Thymidine/metabolism
MH  - *Water Supply
EDAT- 2006/02/10 09:00
MHDA- 2006/04/28 09:00
CRDT- 2006/02/10 09:00
PHST- 2006/02/10 09:00 [pubmed]
PHST- 2006/04/28 09:00 [medline]
PHST- 2006/02/10 09:00 [entrez]
AID - FEM054 [pii]
AID - 10.1111/j.1574-6941.2005.00054.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2006 Mar;55(3):432-8. doi: 10.1111/j.1574-6941.2005.00054.x.