PMID- 16467170
OWN - NLM
STAT- MEDLINE
DCOM- 20060626
LR  - 20181113
IS  - 0021-9746 (Print)
IS  - 1472-4146 (Electronic)
IS  - 0021-9746 (Linking)
VI  - 59
IP  - 4
DP  - 2006 Apr
TI  - Nuclear beta-catenin and Ki-67 expression in choriocarcinoma and its 
      pre-malignant form.
PG  - 387-92
AB  - OBJECTIVE: To study the expression of nuclear beta-catenin and Ki-67 in patients 
      with normal gestation products (NGP), complete hydatidiform moles (CHM), and 
      choriocarcinoma to elucidate their roles in carcinogenesis and their 
      interrelations. METHODS: Expression of nuclear beta-catenin and Ki-67 was studied 
      by immunohistochemistry using paraffin embedded blocks. Sixty NGP, 60 CHM, and 10 
      choriocarcinomas were analysed. In addition, approximately 400 trophoblasts each 
      in 40 NGP, 40 CHM, and 10 choriocarcinomas from the same batch of samples were 
      microdissected for quantitative reverse transcriptase polymerase chain reaction 
      (Q-RT-PCR) to compare beta-catenin mRNA concentration among them. RESULTS: In the 
      chorionic villi of NGP, beta-catenin was consistently expressed in the nuclei of 
      cytotrophoblasts but not syncytiotrophoblasts. Nuclear beta-catenin expression 
      was comparatively reduced in CHM trophoblasts and was absent in choriocarcinoma. 
      By contrast, Ki-67 expression was increased from cytotrophoblasts but not in 
      syncytiotrophoblasts in the chorionic villi of NGP to CHM trophoblasts and 
      choriocarcinoma. Using Q-RT-PCR, beta-catenin mRNA was detected in 10 NGP, 13 
      CHM, and three choriocarcinoma specimens, with median copy numbers of 43,230, 
      18,229, and 17,334 per 400 trophoblasts, respectively. A housekeeping gene 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA was detected as a control 
      in the NGP, CHM, and choriocarcinoma specimens, with median copy numbers of 
      51,300, 54,270, and 97,150 per 400 trophoblasts, respectively. Thus median 
      beta-catenin mRNA values after normalisation were 0.85 in NGP (n = 10), 0.31 in 
      CHM (n = 13), and 0.16 in choriocarcinoma (n = 3). CONCLUSIONS: Decreased nuclear 
      beta-catenin expression and increased Ki-67 expression may be involved in 
      choriocarcinoma carcinogenesis. The findings also suggest that nuclear 
      beta-catenin may play a role in trophoblast differentiation during normal 
      placental development.
FAU - Wong, S C C
AU  - Wong SC
AD  - Department of Clinical Oncology, The Chinese University of Hong Kong, Prince of 
      Wales Hospital, Hong Kong Special Administrative Region, China.
FAU - Chan, A T C
AU  - Chan AT
FAU - Chan, J K C
AU  - Chan JK
FAU - Lo, Y M D
AU  - Lo YM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060207
PL  - England
TA  - J Clin Pathol
JT  - Journal of clinical pathology
JID - 0376601
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (RNA, Messenger)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Biomarkers, Tumor/*analysis
MH  - Cell Nucleus/*chemistry
MH  - Choriocarcinoma/*chemistry
MH  - Chorionic Villi/chemistry
MH  - Embryonic Induction
MH  - Female
MH  - Humans
MH  - Hydatidiform Mole/chemistry
MH  - Immunohistochemistry/methods
MH  - Ki-67 Antigen/*analysis
MH  - Paraffin Embedding
MH  - Precancerous Conditions/metabolism
MH  - Pregnancy
MH  - RNA, Messenger/analysis
MH  - Retrospective Studies
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trophoblasts/chemistry
MH  - Uterine Neoplasms/*chemistry
MH  - Uterus/chemistry
MH  - beta Catenin/*analysis/genetics
PMC - PMC1860375
EDAT- 2006/02/10 09:00
MHDA- 2006/06/27 09:00
PMCR- 2009/04/01
CRDT- 2006/02/10 09:00
PHST- 2006/02/10 09:00 [pubmed]
PHST- 2006/06/27 09:00 [medline]
PHST- 2006/02/10 09:00 [entrez]
PHST- 2009/04/01 00:00 [pmc-release]
AID - jcp.2005.026666 [pii]
AID - cp26666 [pii]
AID - 10.1136/jcp.2005.026666 [doi]
PST - ppublish
SO  - J Clin Pathol. 2006 Apr;59(4):387-92. doi: 10.1136/jcp.2005.026666. Epub 2006 Feb 
      7.