PMID- 16469507
OWN - NLM
STAT- MEDLINE
DCOM- 20070911
LR  - 20131121
IS  - 1050-4648 (Print)
IS  - 1050-4648 (Linking)
VI  - 21
IP  - 2
DP  - 2006 Aug
TI  - Quantitative and qualitative assessment of serum- and inflammatory 
      mediator-induced migration of carp (Cyprinus carpio) head kidney neutrophils in 
      vitro.
PG  - 187-98
AB  - A quantitative transmigration system, permitting the harvest of transmigrated 
      cells for further analysis, was used to study carp head kidney (HK) granulocyte 
      migration in vitro. Pooled carp serum and leukotriene B4 (LTB-4), but not 
      recombinant human C-X-C chemokine ligand 8 (rhCXCL8), recombinant human 
      complement component 5a (rhC5a) or N-formyl-methionyl-leucyl-phenylalanine (FMLP) 
      induced strong migration (up to 70%) of carp HK granulocytes. The transmigrated 
      cells were viable (>or=96%) and uniform (purity >or=97%). After serum- as well as 
      LTB-4-induced transmigration granulocytes produced the same amounts of reactive 
      oxygen species (ROS) as non-migrated cells in HK cell suspension. Their 
      morphology, staining characteristics and flow cytometric scatter characteristics, 
      plus their ability to produce ROS characterised the transmigrated granulocytes as 
      neutrophils. The quantitative transmigration system described here could also 
      serve as an excellent tool for the selective attraction and isolation of highly 
      purified carp neutrophils from HK cell suspensions.
FAU - Stakauskas, Rimantas
AU  - Stakauskas R
AD  - Fish Disease Research Unit, University of Veterinary Medicine Hannover, Buenteweg 
      17, D-30559 Hannover, Germany. rimantas.stakauskas@tiho-hannover.de
FAU - Steinhagen, Dieter
AU  - Steinhagen D
FAU - Guzys, Giedrius
AU  - Guzys G
FAU - Mironova, Lilija
AU  - Mironova L
FAU - Leibold, Wolfgang
AU  - Leibold W
FAU - Schuberth, Hans-Joachim
AU  - Schuberth HJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20060208
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Reactive Oxygen Species)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 80295-54-1 (Complement C5a)
SB  - IM
MH  - Animals
MH  - Carps/immunology/*physiology
MH  - Cattle
MH  - Cells, Cultured
MH  - Chemotaxis, Leukocyte/*physiology
MH  - Complement C5a/physiology
MH  - Flow Cytometry/methods
MH  - Humans
MH  - Leukotriene B4/*physiology
MH  - N-Formylmethionine Leucyl-Phenylalanine/metabolism
MH  - Neutrophils/*physiology
MH  - Reactive Oxygen Species/analysis
MH  - Serum/physiology
MH  - Time Factors
EDAT- 2006/02/14 09:00
MHDA- 2007/09/12 09:00
CRDT- 2006/02/14 09:00
PHST- 2005/08/29 00:00 [received]
PHST- 2005/11/07 00:00 [revised]
PHST- 2005/11/11 00:00 [accepted]
PHST- 2006/02/14 09:00 [pubmed]
PHST- 2007/09/12 09:00 [medline]
PHST- 2006/02/14 09:00 [entrez]
AID - S1050-4648(05)00216-0 [pii]
AID - 10.1016/j.fsi.2005.11.007 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2006 Aug;21(2):187-98. doi: 10.1016/j.fsi.2005.11.007. 
      Epub 2006 Feb 8.