PMID- 16470677
OWN - NLM
STAT- MEDLINE
DCOM- 20070723
LR  - 20151119
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 26
IP  - 2
DP  - 2006 Feb
TI  - Fetal microchimerism is not involved in the pathogenesis of lichen sclerosus of 
      the vulva.
PG  - 175-8
AB  - OBJECTIVES: The aim of this study was to investigate a possible relationship 
      between fetal cell microchimerism and lichen sclerosus of the vulva. We searched 
      for the presence of male cells and DNA in vulval tissue samples. METHODS: 
      Paraffin-embedded skin biopsy samples from 15 women affected with vulval lichen 
      sclerosus who gave birth to at least one son were analyzed for the presence of 
      microchimeric male cells using fluorescence in situ hybridization (FISH) and 
      fluorescent PCR. We included three lichen sclerosus samples originating from 
      women without male offspring, six vulval specimens without pathological finding 
      originating from autopsies and seven male gingival specimens as controls. 
      RESULTS: Nucleated cells containing Y-chromosome specific sequences were neither 
      detected at any site of the lesions nor in normal vulval specimens by using FISH. 
      These results were confirmed by the use of PCR amplification demonstrating only 
      DNA sequences specific for the X chromosome. No female microchimerism was 
      detected in the male gingival samples. CONCLUSION: Despite the limited number and 
      size of the samples, we conclude that persistent male fetal cells are not 
      involved in the pathogenesis of lichen sclerosus of the vulva, since we 
      consistently could not detect Y-chromosome specific sequences by using two 
      molecular techniques.
CI  - Copyright 2006 John Wiley & Sons, Ltd.
FAU - Bauer, Margit
AU  - Bauer M
AD  - Department of Obstetrics and Gynaecology, Medical University Graz, Austria.
FAU - Weger, Wolfgang
AU  - Weger W
FAU - Orescovic, Irmgard
AU  - Orescovic I
FAU - Hiebaum, Eva Maria
AU  - Hiebaum EM
FAU - Benedicic, Christoph
AU  - Benedicic C
FAU - Lang, Uwe
AU  - Lang U
FAU - Pertl, Christof
AU  - Pertl C
FAU - Pertl, Barbara
AU  - Pertl B
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - *Chimerism
MH  - Female
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant, Newborn
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Skin/chemistry/pathology
MH  - Vulva/chemistry/*pathology
MH  - Vulvar Lichen Sclerosus/*diagnosis/*etiology/immunology
EDAT- 2006/02/14 09:00
MHDA- 2007/07/24 09:00
CRDT- 2006/02/14 09:00
PHST- 2006/02/14 09:00 [pubmed]
PHST- 2007/07/24 09:00 [medline]
PHST- 2006/02/14 09:00 [entrez]
AID - 10.1002/pd.1372 [doi]
PST - ppublish
SO  - Prenat Diagn. 2006 Feb;26(2):175-8. doi: 10.1002/pd.1372.