PMID- 16470921
OWN - NLM
STAT- MEDLINE
DCOM- 20061128
LR  - 20220327
IS  - 1008-9292 (Print)
IS  - 1008-9292 (Linking)
VI  - 35
IP  - 1
DP  - 2006 Jan
TI  - [Apoptosis induces by exogenous nitric oxide in Tca8113 cells].
PG  - 50-4
AB  - OBJECTIVE: To observe the apoptosis induced by exogenous NO in Tca8113 cells and 
      to investigate the possible mechanism. METHODS: SNP as NO donor was used to treat 
      the tongue squamous cell carcinoma Tca8113 cells. Cytotoxic and apoptotic effects 
      of NO on Tca8113 cells were examined by using MTT assay, acridine orange (AO) 
      staining, Wright-Giemsa staining, agarose gel electrophoresis and flow cytometry. 
      Western blot was performed for investigating the apoptotic mechanism. RESULTS: NO 
      had a remarkable proliferation inhibiting effect on Tca8113 cells. After being 
      exposed to exogenous NO, Tca8113 cells showed series of apoptotic morphological 
      changes such as cell shrinkage, nuclear condensation; and also showed DNA 
      fragmentation, G2/M phase arrest as well as upregulation of the tumor suppressor 
      P53 protein. CONCLUSION: Exogenous NO has a proliferation inhibition and 
      apoptosis induction effect on Tca8113 cells in a concentration and time-dependent 
      manner, P53 protein may be involved in the apoptosis induced by NO.
FAU - Dong, Xiao-yan
AU  - Dong XY
AD  - The Affiliated of Stomatological Hospital, College of Medicine, Zhejiang 
      University, Hangzhou 310006, China. tongxy112@sina.com
FAU - Zhao, Shi-fang
AU  - Zhao SF
FAU - Zhu, Fu-dong
AU  - Zhu FD
FAU - Liu, Yan-ming
AU  - Liu YM
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhejiang Da Xue Xue Bao Yi Xue Ban
JT  - Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical 
      sciences
JID - 100927946
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Apoptosis/*drug effects
MH  - Carcinoma, Squamous Cell/metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Humans
MH  - Nitric Oxide/*pharmacology
MH  - Tongue Neoplasms/metabolism/*pathology
MH  - Tumor Suppressor Protein p53/*biosynthesis/genetics
EDAT- 2006/02/14 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/02/14 09:00
PHST- 2006/02/14 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/02/14 09:00 [entrez]
AID - 10.3785/j.issn.1008-9292.2006.01.010 [doi]
PST - ppublish
SO  - Zhejiang Da Xue Xue Bao Yi Xue Ban. 2006 Jan;35(1):50-4. doi: 
      10.3785/j.issn.1008-9292.2006.01.010.