PMID- 16474157
OWN - NLM
STAT- MEDLINE
DCOM- 20060317
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 80
IP  - 5
DP  - 2006 Mar
TI  - Preferential infection of mature dendritic cells by mouse hepatitis virus strain 
      JHM.
PG  - 2506-14
AB  - Mouse hepatitis virus strain JHM (MHV-JHM) causes acute encephalitis and acute 
      and chronic demyelinating diseases in mice. Dendritic cells (DCs) are key cells 
      in the initiation of innate and adaptive immune responses, and infection of these 
      cells could potentially contribute to a dysregulated immune response; consistent 
      with this, recent results suggest that DCs are readily infected by another strain 
      of mouse hepatitis virus, the A59 strain (MHV-A59). Herein, we show that the JHM 
      strain also productively infected DCs. Moreover, mature DCs were at least 10 
      times more susceptible than immature DCs to infection with MHV-JHM. DC function 
      was impaired after MHV-JHM infection, resulting in decreased stimulation of CD8 T 
      cells in vitro. Preferential infection of mature DCs was not due to differential 
      expression of the MHV-JHM receptor CEACAM-1a on mature or immature cells or to 
      differences in apoptosis. Although we could not detect infected DCs in vivo, both 
      CD8(+) and CD11b(+) splenic DCs were susceptible to infection with MHV-JHM 
      directly ex vivo. This preferential infection of mature DCs may inhibit the 
      development of an efficient immune response to the virus.
FAU - Zhou, Haixia
AU  - Zhou H
AD  - Department of Pediatrics, University of Iowa, Iowa City, 52242, USA.
FAU - Perlman, Stanley
AU  - Perlman S
LA  - eng
GR  - R01 NS036592/NS/NINDS NIH HHS/United States
GR  - R01 NS36592/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (Ceacam1 protein, mouse)
RN  - 0 (Receptors, Virus)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Carcinoembryonic Antigen/analysis
MH  - Coronavirus Infections/*immunology/*pathology/virology
MH  - Dendritic Cells/*immunology/*virology
MH  - Disease Models, Animal
MH  - Flow Cytometry
MH  - Genes, Reporter
MH  - Green Fluorescent Proteins/analysis/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Murine hepatitis virus/*growth & development
MH  - Receptors, Virus/analysis
PMC - PMC1395395
EDAT- 2006/02/14 09:00
MHDA- 2006/03/18 09:00
PMCR- 2006/07/01
CRDT- 2006/02/14 09:00
PHST- 2006/02/14 09:00 [pubmed]
PHST- 2006/03/18 09:00 [medline]
PHST- 2006/02/14 09:00 [entrez]
PHST- 2006/07/01 00:00 [pmc-release]
AID - 80/5/2506 [pii]
AID - 1969-05 [pii]
AID - 10.1128/JVI.80.5.2506-2514.2006 [doi]
PST - ppublish
SO  - J Virol. 2006 Mar;80(5):2506-14. doi: 10.1128/JVI.80.5.2506-2514.2006.