PMID- 1647552
OWN - NLM
STAT- MEDLINE
DCOM- 19910729
LR  - 20061115
IS  - 0340-6245 (Print)
IS  - 0340-6245 (Linking)
VI  - 65
IP  - 4
DP  - 1991 Apr 8
TI  - Antithrombotic and anticoagulant activity of depolymerized fragment of the 
      glycosaminoglycan extracted from Stichopus japonicus Selenka.
PG  - 369-73
AB  - The antithrombotic and anticoagulant activities of depolymerized fragment (DHG-1) 
      of glycosaminoglycan extracted from Stichopus japonicus Selenka (FGAG) were 
      compared with those of unfractionated heparin (UFH) or low molecular weight 
      heparin (LMWH). DHG-1 at more than 0.3 mg/kg i.v. significantly prevented death 
      of mice treated with thrombin (800 U/kg i.v.). Under the same conditions, FGAG, 
      UFH and LMWH significantly prevented death of mice at more than 0.3, 0.3 and 0.6 
      mg/kg i.v., respectively. In normal plasma, the concentration required to double 
      the activated partial thromboplastin time (doubling APTT) of DHG-1, FGAG, LMWH 
      and UFH were 12.0, 2.4, 5.8, and 1.2 micrograms/ml, respectively. In antithrombin 
      III (AT III)-depleted plasma, doubling APTT of DHG-1, FGAG, and UFH were 11.3, 
      2.1, and 18.5 micrograms/ml, respectively. Prothrombin activation in 
      contact-activated plasma was inhibited completely for 60 s at doubling APTT by 
      all glycosaminoglycans used in this study. DHG-1, however, showed much less 
      antithrombin activity than UFH as tested by thrombin clotting time in plasma and 
      chromogenic assay in the presence of AT III. Moreover, DHG-1 showed much less 
      inhibitory activity on factor Xa, factor IXa, and glass surface-induced factor 
      IXa generation than UFH. These results suggested that DHG-1 is one of the 
      promising antithrombotic agents with quite different anticoagulant property from 
      UFH or LMWH.
FAU - Suzuki, N
AU  - Suzuki N
AD  - Taiho Pharmaceutical Co., Ltd, Tokushima, Japan.
FAU - Kitazato, K
AU  - Kitazato K
FAU - Takamatsu, J
AU  - Takamatsu J
FAU - Saito, H
AU  - Saito H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Germany
TA  - Thromb Haemost
JT  - Thrombosis and haemostasis
JID - 7608063
RN  - 0 (Anticoagulants)
RN  - 0 (Fibrinolytic Agents)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Animals
MH  - *Anticoagulants/isolation & purification
MH  - Blood Coagulation/drug effects
MH  - Blood Coagulation Tests/methods
MH  - *Fibrinolytic Agents/isolation & purification
MH  - Glycosaminoglycans/isolation & purification/*pharmacology
MH  - Heparin
MH  - Heparin, Low-Molecular-Weight/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Sea Cucumbers
MH  - Thrombosis/prevention & control
EDAT- 1991/04/08 00:00
MHDA- 1991/04/08 00:01
CRDT- 1991/04/08 00:00
PHST- 1991/04/08 00:00 [pubmed]
PHST- 1991/04/08 00:01 [medline]
PHST- 1991/04/08 00:00 [entrez]
PST - ppublish
SO  - Thromb Haemost. 1991 Apr 8;65(4):369-73.