PMID- 16475701
OWN - NLM
STAT- MEDLINE
DCOM- 20060309
LR  - 20171116
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 26
IP  - 1A
DP  - 2006 Jan-Feb
TI  - Cytidine-phosphate-guanosine oligodeoxynucleotides and 
      interferon-alpha-expressing tumor cells effectively induce dendritic cell 
      maturation in vitro.
PG  - 211-8
AB  - BACKGROUND: Dendritic cells (DCs) play an important role in immune response and 
      cytidine-phosphate-guanosine (CpG) oligodeoxynucleotides (ODN) as well as 
      interferon (IFN)-alpha have been proven to induce DC maturation. In this study, 
      the synergistic effects of CpG-ODN and IFN-alpha on DC maturation were evaluated. 
      MATERIALS AND METHODS: Surface molecules on DCs and the stimulatory responses of 
      DCs to allogeneic splenocytes were analyzed after cultivation with CpG-ODN and 
      IFN-alpha-overexpressing murine colorectal cancer MC38 cells (MC38-IFNalpha). 
      RESULTS: Co-incubation with CpG-ODN and MC38-IFNalpha, but not wild-type MC38 
      cells (MC38-WT), effectively up-regulated co-stimulatory molecules on the DCs. 
      CpG, in combination with IFN-alpha, stimulated IL-1beta and TNF-alpha production 
      by DCs effectively. When DCs preincubated with CpG-ODN and MC38-IFNalpha were 
      co-incubated with allogeneic splenocytes in vitro, the proliferation of these 
      splenocytes was significantly enhanced compared with that of splenocytes 
      incubated with CpG-ODN and MC38-WT cells (p = 0.041). CONCLUSION: Since CpG-ODN 
      and IFN-alpha have synergistic effects on DC maturation, they may induce potent 
      antitumor immune responses and combination therapy should be considered for 
      clinical application.
FAU - Hiraide, Ayako
AU  - Hiraide A
AD  - Second Department of Internal Medicine, Showa University School of Medicine, 
      Shinagawa-ku, Tokyo 142-8666, Japan.
FAU - Hiroishi, Kazumasa
AU  - Hiroishi K
FAU - Ishii, Shigeaki
AU  - Ishii S
FAU - Eguchi, Junichi
AU  - Eguchi J
FAU - Imawari, Michio
AU  - Imawari M
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Interferon-alpha)
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - Animals
MH  - B7-1 Antigen/biosynthesis/immunology
MH  - B7-2 Antigen/biosynthesis/immunology
MH  - Coculture Techniques
MH  - Colorectal Neoplasms/genetics/immunology/pathology
MH  - CpG Islands/genetics/*immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - Female
MH  - Interferon-alpha/biosynthesis/genetics/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Oligonucleotides/biosynthesis/genetics/*immunology
MH  - Spleen/cytology/immunology
MH  - Up-Regulation
EDAT- 2006/02/16 09:00
MHDA- 2006/03/10 09:00
CRDT- 2006/02/16 09:00
PHST- 2006/02/16 09:00 [pubmed]
PHST- 2006/03/10 09:00 [medline]
PHST- 2006/02/16 09:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2006 Jan-Feb;26(1A):211-8.