PMID- 16475713
OWN - NLM
STAT- MEDLINE
DCOM- 20060309
LR  - 20140729
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 26
IP  - 1A
DP  - 2006 Jan-Feb
TI  - Sex chromosome abnormalities in bladder cancer: Y polysomies are linked to 
      PT1-grade III transitional cell carcinoma.
PG  - 319-23
AB  - BACKGROUND: Bladder cancer is a heterogeneous group of tumors from both the 
      biological and clinical points of view. Conventional cytogenetics and molecular 
      genetic techniques have shown non-random aberrations in bladder cancer, while 
      certain chromosomal changes have been found to be highly correlated with tumor 
      grade or stage. The aim of this study was to evaluate, by fluorescence in situ 
      hybridization (FISH), the numerical aberrations of chromosomes X and Y in bladder 
      cancer, comparing the incidence of nuclei with aneusomies in different grades or 
      histological stages of the tumors. MATERIALS AND METHODS: The FISH technique, 
      using DNA probes specific for chromosomes X and Y, was applied to 35 male bladder 
      tumor specimens directly processed for cytogenetic study. RESULTS: Polysomies of 
      chromosome X were observed in 25 out of the 35 cases examined (71.43%), while 
      numerical aberrations of chromosome Y were observed in 22 out of the 35 cases 
      (62.86%). Of those cases with numerical aberrations of chromosome Y, 13 had 
      polysomy (37.14%), while in 9 cases, loss of Y was observed (25.71%). Statistical 
      analysis showed that numerical aberrations on chromosomes X or Y were not linked 
      to histological stage, while a probable correlation was observed between 
      aneusomies X or Y and tumor grade. Comparing the results of PT1-grade III tumors 
      with those of PT1-grade II, statistical analysis showed that aneusomies Y and, 
      especially, polysomy Y were correlated with PT1-grade III tumors, p = 0.023 and p 
      = 0.010, respectively. An uncertain correlation between polysomy X and PT1-grade 
      III tumors was found, p = 0.070. CONCLUSION: Our results may suggest that the 
      genetic instability associated with PT1-grade III tumors may account for the 
      considerable potential for aggression of these tumors. However, to draw definite 
      conclusions, a large number of cases must be studied.
FAU - Panani, Anna D
AU  - Panani AD
AD  - Critical Care Department, Medical School of Athens University, Research Unit, 
      Evangelismos Hospital, Ipsilandou 45-47, Athens 10676, Greece. apanani@med.uoa.gr
FAU - Roussos, Charis
AU  - Roussos C
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
SB  - IM
MH  - Aneuploidy
MH  - Carcinoma, Transitional Cell/*genetics/pathology
MH  - Chromosomes, Human, X
MH  - *Chromosomes, Human, Y
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Sex Chromosome Aberrations
MH  - Urinary Bladder Neoplasms/*genetics/pathology
EDAT- 2006/02/16 09:00
MHDA- 2006/03/10 09:00
CRDT- 2006/02/16 09:00
PHST- 2006/02/16 09:00 [pubmed]
PHST- 2006/03/10 09:00 [medline]
PHST- 2006/02/16 09:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2006 Jan-Feb;26(1A):319-23.