PMID- 16476971
OWN - NLM
STAT- MEDLINE
DCOM- 20060330
LR  - 20201006
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 87
IP  - Pt 3
DP  - 2006 Mar
TI  - Human herpesvirus 7 U47 gene products are glycoproteins expressed in virions and 
      associate with glycoprotein H.
PG  - 501-508
LID - 10.1099/vir.0.81374-0 [doi]
AB  - The function of the human herpesvirus 7 (HHV-7) U47 gene, which is a positional 
      homologue of the genes encoding glycoprotein O (gO) in human cytomegalovirus 
      (HCMV) and human herpesvirus 6 (HHV-6), was analysed. A monoclonal antibody (mAb) 
      against the U47 gene product reacted in immunoblots with proteins migrating at 49 
      and 51 kDa in lysates of HHV-7-infected cells and with 49 and 51 kDa proteins in 
      partially purified virions. Digestion of the 49 and 51 kDa proteins with 
      endoglycosidase H and peptide N-glycosidase F indicated that the U47-encoded 
      proteins were modified with N-linked oligosaccharides. Therefore, the U47 gene 
      and its product were named gO, as in HCMV and HHV-6. In addition, the anti-gO mAb 
      co-immunoprecipitated glycoprotein H (gH) in HHV-7-infected cells, indicating an 
      association between HHV-7 gO and gH. The results suggest that the HHV-7 gO-gH 
      complex might have a similar function to that in HCMV or HHV-6, such as cell-cell 
      fusion in virus infection.
FAU - Sadaoka, Tomohiko
AU  - Sadaoka T
AD  - Laboratory of Virology and Vaccinology, Division of Biomedical Research, National 
      Institute of Biomedical Innovation, 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, 
      Japan.
FAU - Yamanishi, Koichi
AU  - Yamanishi K
AD  - Laboratory of Virology and Vaccinology, Division of Biomedical Research, National 
      Institute of Biomedical Innovation, 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, 
      Japan.
FAU - Mori, Yasuko
AU  - Mori Y
AD  - Laboratory of Virology and Vaccinology, Division of Biomedical Research, National 
      Institute of Biomedical Innovation, 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, 
      Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Blotting, Western
MH  - Cell Line
MH  - Fluorescent Antibody Technique
MH  - Glycoproteins/chemistry/*metabolism
MH  - Herpesvirus 7, Human/*chemistry
MH  - Humans
MH  - Molecular Weight
MH  - Viral Envelope Proteins/chemistry/*metabolism
MH  - Virion/*metabolism
EDAT- 2006/02/16 09:00
MHDA- 2006/03/31 09:00
CRDT- 2006/02/16 09:00
PHST- 2006/02/16 09:00 [pubmed]
PHST- 2006/03/31 09:00 [medline]
PHST- 2006/02/16 09:00 [entrez]
AID - 10.1099/vir.0.81374-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2006 Mar;87(Pt 3):501-508. doi: 10.1099/vir.0.81374-0.