PMID- 16477384
OWN - NLM
STAT- MEDLINE
DCOM- 20060713
LR  - 20181201
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 284
IP  - 1-2
DP  - 2006 Mar
TI  - Modulation of DNA intercalation by resveratrol and genistein.
PG  - 57-64
AB  - Time correlated Single Photon Counting study (TCSPC) was performed for the first 
      time to evaluate the effect of resveratrol (RES) and genistein (GEN) at 10-100 
      microM and 10-150 microM respectively, in modulating the DNA conformation and the 
      variation induced due to intercalation by the dyes, ethidium bromide (EtBr) and 
      acridine orange (AO). It is demonstrated using UV-absorption and fluorescence 
      spectroscopy that RES and GEN, at 50 microM and 100 microM respectively can bind 
      to DNA resulting in significant de-intercalation of the dyes, preventing their 
      further intercalation within DNA. Hyperchromicity with red/blue shifts in DNA 
      when bound to dyes was reduced upon addition of RES and GEN. DNA-dependent 
      fluorescence of EtBr and AO was quenched in the presence of RES by 87.97% and 
      79.13% respectively, while similar quenching effect was observed for these when 
      interacted with GEN (85.52% and 83.85%). It is found from TCSPC analysis that the 
      higher lifetime component or constituent of intercalated dyes (tau(2), A (2)) 
      decreased with the subsequent increase in smaller component or constituent of 
      free dye (tau(1), A (1)) after the interaction of drugs with the intercalated 
      DNA. Thus these findings signify that RES and GEN can play an important role in 
      modulating DNA intercalation, leading to the reduction in DNA-directed toxicity.
FAU - Usha, Subbiah
AU  - Usha S
AD  - Department of Genetics, Dr. ALM Post Graduate Institute of Basic Medical 
      Sciences, University of Madras, Taramani, Chennai 600 113, India.
FAU - Johnson, Irudayam Maria
AU  - Johnson IM
FAU - Malathi, Raghunathan
AU  - Malathi R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Anticarcinogenic Agents)
RN  - 0 (Coloring Agents)
RN  - 0 (Intercalating Agents)
RN  - 0 (Stilbenes)
RN  - 9007-49-2 (DNA)
RN  - 91080-16-9 (calf thymus DNA)
RN  - DH2M523P0H (Genistein)
RN  - EN464416SI (Ethidium)
RN  - F30N4O6XVV (Acridine Orange)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Acridine Orange/chemistry
MH  - Anticarcinogenic Agents/*chemistry
MH  - Coloring Agents/chemistry
MH  - DNA/*chemistry
MH  - DNA Damage
MH  - Ethidium/chemistry
MH  - Genistein/*chemistry
MH  - Intercalating Agents/*chemistry
MH  - Nucleic Acid Conformation
MH  - Resveratrol
MH  - Spectrometry, Fluorescence
MH  - Spectrophotometry, Ultraviolet
MH  - Stilbenes/*chemistry
EDAT- 2006/02/16 09:00
MHDA- 2006/07/14 09:00
CRDT- 2006/02/16 09:00
PHST- 2006/02/16 09:00 [pubmed]
PHST- 2006/07/14 09:00 [medline]
PHST- 2006/02/16 09:00 [entrez]
AID - 10.1007/s11010-005-9013-6 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2006 Mar;284(1-2):57-64. doi: 10.1007/s11010-005-9013-6.