PMID- 16477614
OWN - NLM
STAT- MEDLINE
DCOM- 20060601
LR  - 20181201
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 83
IP  - 5
DP  - 2006 Apr
TI  - Trkb receptors modulation of glutamate release is limited to a subset of nerve 
      terminals in the adult rat hippocampus.
PG  - 832-44
AB  - Brain-derived neurotrophic factor (BDNF) modulates glutamatergic excitatory 
      transmission in hippocampal primary cultures by acting at a presynaptic locus. 
      Although it has been suggested that BDNF also modulates adult hippocampus 
      glutamatergic transmission, this remains a matter of controversy. To clarify a 
      putative role for this neurotrophin in the modulation of glutamate release we 
      applied exogenous BDNF to isolated adult rat hippocampal nerve terminals. BDNF, 
      at 100 ng/ml, potentiated by 25% the K(+)-evoked release of [(3)H]glutamate from 
      hippocampal synaptosomes. The small effect of BDNF on [(3)H]glutamate release 
      correlated with a modest increase in phospholipase Cgamma (PLCgamma) 
      phosphorylation, and with the lack of effect of BDNF on extracellular-signal 
      regulated kinase (ERK) and Akt phosphorylation. Immunocytochemistry studies 
      demonstrated that only about one-third of glutamatergic synaptosomes were 
      positive for TrkB immunoreactivity. Furthermore, biotinylation and subsynaptic 
      fractionation studies showed that only one-fourth of total full-length TrkB was 
      present at the plasma membrane, evenly distributed between the presynaptic active 
      zone and the postsynaptic density. These results indicate that BDNF modulates 
      synaptic transmission presynaptically in a small subset of hippocampal 
      glutamatergic synapses that contain TrkB and that express the receptor on the 
      plasma membrane.
FAU - Pereira, Daniela B
AU  - Pereira DB
AD  - Department of Zoology, Center for Neuroscience and Cell Biology, University of 
      Coimbra, Coimbra, Portugal.
FAU - Rebola, Nelson
AU  - Rebola N
FAU - Rodrigues, Ricardo J
AU  - Rodrigues RJ
FAU - Cunha, Rodrigo A
AU  - Cunha RA
FAU - Carvalho, Arselio P
AU  - Carvalho AP
FAU - Duarte, Carlos B
AU  - Duarte CB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism/pharmacology
MH  - Cells, Cultured
MH  - Extracellular Signal-Regulated MAP Kinases/drug effects/metabolism
MH  - Glutamic Acid/*metabolism
MH  - Hippocampus/drug effects/*metabolism
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Male
MH  - Presynaptic Terminals/drug effects/*metabolism
MH  - Proto-Oncogene Proteins c-akt/drug effects/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/*metabolism
MH  - Synaptosomes/drug effects/metabolism
EDAT- 2006/02/16 09:00
MHDA- 2006/06/02 09:00
CRDT- 2006/02/16 09:00
PHST- 2006/02/16 09:00 [pubmed]
PHST- 2006/06/02 09:00 [medline]
PHST- 2006/02/16 09:00 [entrez]
AID - 10.1002/jnr.20784 [doi]
PST - ppublish
SO  - J Neurosci Res. 2006 Apr;83(5):832-44. doi: 10.1002/jnr.20784.