PMID- 16477661
OWN - NLM
STAT- MEDLINE
DCOM- 20060808
LR  - 20060605
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 94
IP  - 4
DP  - 2006 Jul 5
TI  - Community structures and activities of nitrifying and denitrifying bacteria in 
      industrial wastewater-treating biofilms.
PG  - 762-72
AB  - The bacterial community structure, in situ spatial distributions and activities 
      of nitrifying and denitrifying bacteria in biofilms treating industrial 
      wastewater were investigated by combination of the 16S rRNA gene clone analysis, 
      fluorescence in situ hybridization (FISH) and microelectrodes. These results were 
      compared with the nitrogen removal capacity of the industrial wastewater 
      treatment plant (IWTP). Both nitrification and denitrification occurred in the 
      primary denitrification (PD) tank and denitrification occurred in the secondary 
      denitrification (SD) tank. In contrast, nitrification and denitrification rates 
      were very low in the nitrification (N) tank. 16S rRNA gene clone sequence 
      analysis revealed that the bacteria affiliated with Alphaproteobacteria, followed 
      by Betaproteobacteria, were numerically important microbial groups in three 
      tanks. The many clones affiliated with Alphaproteobacteria were closely related 
      to the denitrifying bacteria (e.g., Hyphomicrobium spp., Rhodopseudomonas 
      palustris, and Rhodobacter spp.). In addition, Methylophilus leisingeri 
      affiliated with Betaproteobacteria, which favorably utilized methanol, was 
      detected only in the SD-tank to which methanol was added. Nitrosomonas europaea 
      and Nitrosomonas marina were detected as the ammonia-oxidizing bacteria 
      affiliated with Betaproteobacteria throughout this plant, although the dominant 
      species of them was different among three tanks. Nitrifying bacteria were mainly 
      detected in the upper parts of the PD-biofilm whereas their populations were low 
      in the upper parts of the N-biofilm. The presence of denitrifying bacteria 
      affiliated with Hyphomicrobium spp. in SD- and N-biofilms was verified by FISH 
      analysis. Microelectrode measurements showed that the nitrifying bacteria present 
      in the N- and PD-biofilms were active and the bacteria present in the SD-biofilm 
      could denitrify.
CI  - (c) 2006 Wiley Periodicals, Inc.
FAU - Satoh, Hisashi
AU  - Satoh H
AD  - Department of Environmental and Civil Engineering, Hachinohe Institute of 
      Technology, Hachinohe, Aomori, Japan.
FAU - Yamakawa, Takeshi
AU  - Yamakawa T
FAU - Kindaichi, Tomonori
AU  - Kindaichi T
FAU - Ito, Tsukasa
AU  - Ito T
FAU - Okabe, Satoshi
AU  - Okabe S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (DNA Primers)
RN  - 0 (Nitrites)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacteria/classification/genetics/*isolation & purification/metabolism
MH  - *Biofilms
MH  - DNA Primers
MH  - Electrochemistry/methods
MH  - Electrodes
MH  - Japan
MH  - Microchemistry/methods
MH  - Nitrites/*metabolism
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - RNA, Bacterial/genetics/isolation & purification
MH  - RNA, Ribosomal, 16S/genetics/isolation & purification
MH  - Waste Disposal, Fluid/*instrumentation/methods
MH  - *Water Microbiology
EDAT- 2006/02/16 09:00
MHDA- 2006/08/09 09:00
CRDT- 2006/02/16 09:00
PHST- 2006/02/16 09:00 [pubmed]
PHST- 2006/08/09 09:00 [medline]
PHST- 2006/02/16 09:00 [entrez]
AID - 10.1002/bit.20894 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2006 Jul 5;94(4):762-72. doi: 10.1002/bit.20894.