PMID- 16478447
OWN - NLM
STAT- MEDLINE
DCOM- 20060627
LR  - 20131121
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 8
IP  - 3
DP  - 2006 Mar
TI  - Selective enrichment and molecular characterization of a previously uncultured 
      Nitrospira-like bacterium from activated sludge.
PG  - 405-15
AB  - Previously uncultured nitrite-oxidizing bacteria affiliated to the genus 
      Nitrospira have for the first time been successfully enriched from activated 
      sludge from a municipal wastewater treatment plant. During the enrichment 
      procedure, the abundance of the Nitrospira-like bacteria increased to 
      approximately 86% of the total bacterial population. This high degree of 
      purification was achieved by a novel enrichment protocol, which exploits 
      physiological features of Nitrospira-like bacteria and includes the selective 
      repression of coexisting Nitrobacter cells and heterotrophic contaminants by 
      application of ampicillin in a final concentration of 50 microg ml(-1). The 
      enrichment process was monitored by electron microscopy, fluorescence in situ 
      hybridization (FISH) with rRNA-targeted probes and fatty acid profiling. 
      Phylogenetic analysis of 16S rRNA gene sequences revealed that the enriched 
      bacteria represent a novel Nitrospira species closely related to uncultured 
      Nitrospira-like bacteria previously found in wastewater treatment plants and 
      nitrifying bioreactors. The enriched strain is provisionally classified as 
      'Candidatus Nitrospira defluvii'.
FAU - Spieck, Eva
AU  - Spieck E
AD  - Universitat Hamburg, Biozentrum Klein Flottbek, Mikrobiologie, Ohnhorststr. 18, 
      D-22609 Hamburg, Germany. spieck@mikrobiologie.uni-hamburg.de
FAU - Hartwig, Christine
AU  - Hartwig C
FAU - McCormack, Iris
AU  - McCormack I
FAU - Maixner, Frank
AU  - Maixner F
FAU - Wagner, Michael
AU  - Wagner M
FAU - Lipski, Andre
AU  - Lipski A
FAU - Daims, Holger
AU  - Daims H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Fatty Acids)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 7C782967RD (Ampicillin)
SB  - IM
MH  - Ampicillin/pharmacology
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bacteria/classification/*genetics/*isolation & purification
MH  - Bacterial Physiological Phenomena
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Fatty Acids/analysis/chemistry
MH  - Genes, rRNA
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Electron
MH  - Nitrobacter/drug effects
MH  - RNA, Bacterial/analysis
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
MH  - Water Microbiology
EDAT- 2006/02/16 09:00
MHDA- 2006/06/28 09:00
CRDT- 2006/02/16 09:00
PHST- 2006/02/16 09:00 [pubmed]
PHST- 2006/06/28 09:00 [medline]
PHST- 2006/02/16 09:00 [entrez]
AID - EMI905 [pii]
AID - 10.1111/j.1462-2920.2005.00905.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2006 Mar;8(3):405-15. doi: 10.1111/j.1462-2920.2005.00905.x.