PMID- 16478532
OWN - NLM
STAT- MEDLINE
DCOM- 20060511
LR  - 20061115
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 96
IP  - 5
DP  - 2006 Mar
TI  - Absence of synapsin I and II is accompanied by decreases in vesicular transport 
      of specific neurotransmitters.
PG  - 1458-66
AB  - Studies of synapsin-deficient mice have shown decreases in the number of synaptic 
      vesicles but knowledge about the consequences of this decrease, and which classes 
      of vesicles are being affected, has been lacking. In this study, glutamatergic, 
      GABAergic and dopaminergic transport has been analysed in animals where the genes 
      encoding synapsin I and II were inactivated. The levels of the vesicular 
      glutamate transporter (VGLUT) 1, VGLUT2 and the vesicular GABA transporter (VGAT) 
      were decreased by approximately 40% in adult forebrain from mice devoid of 
      synapsin I and II, while vesicular monoamine transporter (VMAT) 2 and VGLUT3 were 
      present in unchanged amounts compared with wild-type mice. Functional studies on 
      synaptic vesicles showed that the vesicular uptake of glutamate and GABA was 
      decreased by 41 and 23%, respectively, while uptake of dopamine was unaffected by 
      the lack of synapsin I and II. Double-labelling studies showed that VGLUT1 and 
      VGLUT2 colocalized fully with synapsin I and/or II in the hippocampus and 
      neostriatum, respectively. VGAT showed partial colocalization, while VGLUT3 and 
      VMAT2 did not colocalize with either synapsin I or II in the brain areas studied. 
      In conclusion, distinct vesicular transporters show a variable degree of 
      colocalization with synapsin proteins and, hence, distinct sensitivities to 
      inactivation of the genes encoding synapsin I and II.
FAU - Bogen, Inger Lise
AU  - Bogen IL
AD  - Department of Biochemistry, Institute of Basic Medical Sciences, University of 
      Oslo, Oslo, Norway. i.l.bogen@medisin.uio.no
FAU - Boulland, Jean-Luc
AU  - Boulland JL
FAU - Mariussen, Espen
AU  - Mariussen E
FAU - Wright, Marianne S
AU  - Wright MS
FAU - Fonnum, Frode
AU  - Fonnum F
FAU - Kao, Hung-Teh
AU  - Kao HT
FAU - Walaas, S Ivar
AU  - Walaas SI
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (RNA, Messenger)
RN  - 0 (Synapsins)
RN  - 0 (Vesicular Neurotransmitter Transport Proteins)
SB  - IM
MH  - Animals
MH  - Fluorescent Antibody Technique/methods
MH  - Gene Expression/genetics
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Microscopy, Confocal/methods
MH  - Neurotransmitter Agents/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Subcellular Fractions/metabolism
MH  - Synapsins/deficiency/*physiology
MH  - Synaptosomes/metabolism
MH  - Vesicular Neurotransmitter Transport Proteins/classification/*metabolism
EDAT- 2006/02/16 09:00
MHDA- 2006/05/12 09:00
CRDT- 2006/02/16 09:00
PHST- 2006/02/16 09:00 [pubmed]
PHST- 2006/05/12 09:00 [medline]
PHST- 2006/02/16 09:00 [entrez]
AID - JNC3636 [pii]
AID - 10.1111/j.1471-4159.2005.03636.x [doi]
PST - ppublish
SO  - J Neurochem. 2006 Mar;96(5):1458-66. doi: 10.1111/j.1471-4159.2005.03636.x.