PMID- 16478883
OWN - NLM
STAT- MEDLINE
DCOM- 20070817
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 108
IP  - 1
DP  - 2006 Jul 1
TI  - Allogeneic T cells induce rapid CD34+ cell differentiation into CD11c+CD86+ cells 
      with direct and indirect antigen-presenting function.
PG  - 203-8
AB  - Dendritic cells (DCs) derive from CD34+ cells or monocytes and stimulate 
      alloimmune responses in transplantation. We hypothesized that the interaction 
      between CD34+ cells and allogeneic T cells would influence the function of 
      hematopoietic stem cells (HSCs). Cord blood (CB) CD34+ cells proliferated briskly 
      in response to allogeneic, but not autologous, T cells when mixed with irradiated 
      T cells for 6 days in vitro. This proliferation was significantly inhibited by an 
      anti-HLA class II monoclonal antibody (mAb), by an anti-TNFalpha mAb, or by 
      CTLA4-Ig. Allogeneic T cells induced the differentiation of CD34+ progenitors 
      into cells with the morphology of dendritic monocytic precursors and 
      characterized by the expression of HLA-DR, CD86, CD40, CD14, and CD11c, due to an 
      endogenous release of TNFalpha. Cotransplantation of CD34+ cells with allogeneic 
      T cells into nonobese diabetic-severe combined immunodeficiency (NOD/SCID) mice 
      resulted in a greater engraftment of myeloid CD1c+ dendritic cells compared with 
      cotransplantation with autologous T cells. In vitro, CD34+ cell-derived 
      antigen-presenting cells (APCs) were functionally capable of both direct and 
      indirect presentation of alloantigens. Based on these findings, we hypothesize 
      that in HSC transplantation the initial cross talk between allogeneic T cells and 
      CD34+ cells may result in the increased generation of APCs that can present host 
      alloantigens and possibly contribute to the development of graft-versus-host 
      disease.
FAU - Abbasian, Javaneh
AU  - Abbasian J
AD  - Section Hematology/Oncology, University of Illinois at Chicago, Chicago, IL 
      60607-7171, USA.
FAU - Mahmud, Dolores
AU  - Mahmud D
FAU - Mahmud, Nadim
AU  - Mahmud N
FAU - Chunduri, Sandeep
AU  - Chunduri S
FAU - Araki, Hiroto
AU  - Araki H
FAU - Reddy, Pavan
AU  - Reddy P
FAU - Hoffman, Ronald
AU  - Hoffman R
FAU - Arpinati, Mario
AU  - Arpinati M
FAU - Ferrara, James L M
AU  - Ferrara JL
FAU - Rondelli, Damiano
AU  - Rondelli D
LA  - eng
GR  - P01 CA39542/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060214
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD34)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD11c Antigen)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/cytology/*immunology
MH  - Antigens, CD34/*biosynthesis/drug effects
MH  - B7-2 Antigen/*biosynthesis
MH  - CD11c Antigen/*biosynthesis
MH  - Cell Differentiation/drug effects/*immunology
MH  - Cell Proliferation
MH  - Fetal Blood/cytology/*immunology
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Mice
MH  - Mice, SCID
MH  - Structure-Activity Relationship
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Tumor Necrosis Factor-alpha/pharmacology
PMC - PMC1895833
EDAT- 2006/02/16 09:00
MHDA- 2007/08/19 09:00
PMCR- 2007/07/01
CRDT- 2006/02/16 09:00
PHST- 2006/02/16 09:00 [pubmed]
PHST- 2007/08/19 09:00 [medline]
PHST- 2006/02/16 09:00 [entrez]
PHST- 2007/07/01 00:00 [pmc-release]
AID - S0006-4971(20)64938-2 [pii]
AID - 0203 [pii]
AID - 10.1182/blood-2005-11-4330 [doi]
PST - ppublish
SO  - Blood. 2006 Jul 1;108(1):203-8. doi: 10.1182/blood-2005-11-4330. Epub 2006 Feb 
      14.