PMID- 16479191
OWN - NLM
STAT- MEDLINE
DCOM- 20060928
LR  - 20171116
IS  - 0957-5235 (Print)
IS  - 0957-5235 (Linking)
VI  - 17
IP  - 2
DP  - 2006 Mar
TI  - The effects of unfractionated heparin, low molecular weight heparin and 
      danaparoid on the thromboelastogram (TEG): an in-vitro comparison of standard and 
      heparinase-modified TEGs with conventional coagulation assays.
PG  - 97-104
AB  - To investigate the effects of unfractionated heparin (UFH), low molecular weight 
      heparin (LMWH) and danaparoid (DPD) added to whole blood in vitro on standard and 
      heparinase-modified thromboelastogram (TEG) parameters compared with conventional 
      assays of coagulation. The effects of UFH, LMWH and DPD on standard TEG 
      parameters were compared with the prothrombin time, activated partial 
      thromboplastin time, thrombin time and anti-activated factor X (anti-FXa) 
      activity, at concentrations of these anticoagulants ranging from 0.025 to 1 U/ml. 
      In the second part of the study, the effects of very low concentrations 
      (0.005-0.05 U/ml) of UFH, LMWH and DPD on the difference between standard and 
      heparinase-modified TEG parameters were compared with the prothrombin time, 
      activated partial thromboplastin time, thrombin time and anti-FXa activity. 
      Standard TEG parameters were outside the reference range at lower concentrations 
      of UFH, LMWH and DPD than most conventional coagulation assays were able to 
      detect. Only anti-FXa activity was more sensitive to the presence of these 
      anticoagulants than the standard TEG alone. The lowest concentration of UFH, LMWH 
      and DPD used in this study (0.005 U/ml) caused significant differences between 
      the standard and heparinase-modified alpha-angles of the TEG. In addition, the 
      difference between standard and heparinase-modified TEG parameters distinguished 
      between low concentrations (0.005-0.05 U/ml) of UFH with greater sensitivity than 
      anti-FXa activity, but were less sensitive to LMWH and DPD. The standard TEG is 
      more sensitive to UFH, LMWH and DPD than most conventional coagulation tests, 
      with the exception of anti-FXa activity. Calculation of the difference between 
      standard and heparinase-modified TEG parameters greatly increases the sensitivity 
      of the assay for the effects of these anticoagulants, and is more sensitive to 
      very low quantities of UFH than anti-FXa activity.
FAU - Coppell, Jason A
AU  - Coppell JA
AD  - Haemophilia Centre and Haemostasis Unit, Royal Free Hospital, London, UK. 
      jcoppell@medsch.ucl.ac.uk
FAU - Thalheimer, Ulrich
AU  - Thalheimer U
FAU - Zambruni, Andrea
AU  - Zambruni A
FAU - Triantos, Christos K
AU  - Triantos CK
FAU - Riddell, Anne F
AU  - Riddell AF
FAU - Burroughs, Andrew K
AU  - Burroughs AK
FAU - Perry, David J
AU  - Perry DJ
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Blood Coagul Fibrinolysis
JT  - Blood coagulation & fibrinolysis : an international journal in haemostasis and 
      thrombosis
JID - 9102551
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 24967-94-0 (Dermatan Sulfate)
RN  - 9007-28-7 (Chondroitin Sulfates)
RN  - 9050-30-0 (Heparitin Sulfate)
RN  - BI6GY4U9CW (danaparoid)
RN  - EC 3.4.21.6 (Factor Xa)
RN  - EC 4.2.2.7 (Heparin Lyase)
SB  - IM
MH  - Adult
MH  - Chondroitin Sulfates/*chemistry
MH  - Dermatan Sulfate/*chemistry
MH  - Factor Xa/analysis
MH  - Heparin Lyase/*chemistry
MH  - Heparin, Low-Molecular-Weight/*chemistry
MH  - Heparitin Sulfate/*chemistry
MH  - Humans
MH  - Male
MH  - Partial Thromboplastin Time/methods
MH  - Reference Standards
MH  - Sensitivity and Specificity
MH  - *Thrombelastography/methods
EDAT- 2006/02/16 09:00
MHDA- 2006/09/29 09:00
CRDT- 2006/02/16 09:00
PHST- 2006/02/16 09:00 [pubmed]
PHST- 2006/09/29 09:00 [medline]
PHST- 2006/02/16 09:00 [entrez]
AID - 00001721-200603000-00003 [pii]
AID - 10.1097/01.mbc.0000203859.62739.25 [doi]
PST - ppublish
SO  - Blood Coagul Fibrinolysis. 2006 Mar;17(2):97-104. doi: 
      10.1097/01.mbc.0000203859.62739.25.