PMID- 16479386
OWN - NLM
STAT- MEDLINE
DCOM- 20070130
LR  - 20181113
IS  - 0017-6192 (Print)
IS  - 0017-6192 (Linking)
VI  - 54
IP  - 9
DP  - 2006 Sep
TI  - [Influence of ischemia/hypoxia on the HIF-1 activity and expression of 
      hypoxia-dependent genes in the cochlea of the newborn rat].
PG  - 689-97
AB  - BACKGROUND: Transcription factor HIF-1 (hypoxia-inducible factor-1) regulates the 
      expression of genes which are involved in glucose supply, growth, metabolism, 
      redox reactions and blood supply. Hypoxia and ischemia play an important role in 
      the pathogenesis of tinnitus and hearing loss. Therefore, HIF-1 activity and the 
      expression of HIF-1 dependent genes in the cochlea were examined under ischemic 
      and hypoxic conditions. MATERIAL AND METHODS: For the HIF-1 analysis, single-cell 
      cultures of the organ of Corti (OC), stria vascularis (SV) and modiolus (MOD) 
      were used. mRNA expression was analyzed in the organotypic culture using a 
      microarray technique (RN U34-chip, Affymetrix). RESULTS: Ischemia (hypoxia 
      without glucose) and pure hypoxia increase the HIF-1 activity identically, with 
      the highest increase found in MOD and OC. The HIF-1 alpha mRNA levels were found 
      to be higher in SV than in the OC and MOD. During culturing, there is a clear 
      increase in HIF-1 alpha mRNA and the expression of a number of HIF-1 dependent 
      genes, such as Gapdh/glyceraldehyde-3-phosphate dehydrogenase, Slc2a1/solute 
      carrier family 2 (facilitated glucose transporter), member 1, Tf/transferrin and 
      Tfrc/transferrin receptor, in all three regions. In SV, MOD and OC, increase in 
      the expression of Hmox1/hemoxygenase 1, Nos2/nitric oxide synthase, inducible and 
      Tfrc is particularly high. Hypoxia (5 h) results in an increased expression of 
      Igf2/Insulin-like growth factor 2. CONCLUSION: The present data underline the 
      contribution of radical forming processes to the pathogenesis of inner ear 
      diseases. For experimental research, it is important to note that organotypic 
      culture may be coupled with hypoxia.
FAU - Mazurek, B
AU  - Mazurek B
AD  - Molekularbiologisches Forschungslabor der HNO-Klinik, 
      Charite--Universitatsmedizin Berlin.
FAU - Rheinlander, C
AU  - Rheinlander C
FAU - Fuchs, F-U
AU  - Fuchs FU
FAU - Amarjargal, N
AU  - Amarjargal N
FAU - Kuban, R-J
AU  - Kuban RJ
FAU - Ungethum, U
AU  - Ungethum U
FAU - Haupt, H
AU  - Haupt H
FAU - Kietzmann, T
AU  - Kietzmann T
FAU - Gross, J
AU  - Gross J
LA  - ger
PT  - Journal Article
TT  - Einfluss von Ischamie/Hypoxie auf die HIF-1-Aktivitat und Expression von 
      hypoxieabhangigen Genen in der Kochlea der neugeborenen Ratte.
PL  - Germany
TA  - HNO
JT  - HNO
JID - 2985099R
RN  - 0 (Hypoxia-Inducible Factor 1)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Hypoxia
MH  - Cells, Cultured
MH  - Cochlea/*metabolism
MH  - Gene Expression Regulation
MH  - Hypoxia/*metabolism
MH  - Hypoxia-Inducible Factor 1/*metabolism
MH  - Ischemia/*metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 2006/02/16 09:00
MHDA- 2007/01/31 09:00
CRDT- 2006/02/16 09:00
PHST- 2006/02/16 09:00 [pubmed]
PHST- 2007/01/31 09:00 [medline]
PHST- 2006/02/16 09:00 [entrez]
AID - 10.1007/s00106-005-1371-6 [doi]
PST - ppublish
SO  - HNO. 2006 Sep;54(9):689-97. doi: 10.1007/s00106-005-1371-6.