PMID- 1647969
OWN - NLM
STAT- MEDLINE
DCOM- 19910802
LR  - 20191210
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 284
IP  - 1
DP  - 1991 Jun 17
TI  - Calcium mobilization in human platelets by receptor agonists and calcium-ATPase 
      inhibitors.
PG  - 1-4
AB  - Inhibitors of the endoplasmic reticulum Ca(2+)-ATPase like thapsigargin (TG) and 
      2,5-di (tert-butyl)-1,4-benzohydroquinone (tBuBHQ) cause increases in cytosolic 
      calcium in intact human platelets resulting from prevention of reuptake. A 
      maximal concentration of TG (0.2 microM) mobilized 100% of sequestered Ca2+ 
      compared to the action of a receptor agonist like thrombin (0.1 U/ml). A maximal 
      dose of tBuBHQ (50 microM) stimulated release of about 40% of intracellular 
      calcium compared to thrombin and TG. The reduced ability of tBuBHQ to release 
      calcium can be explained with an inhibitory effect on the cyclooxygenase pathway 
      (Ki approximately 7 microM). Therefore tBuBHQ is not able to cause platelet 
      aggregation compared to TG. In the presence of a cyclooxygenase inhibitor or a 
      thromboxane A2 receptor antagonist the action of TG is identical to that observed 
      with tBuBHQ. Generally, inhibition of calcium sequestration does not 
      automatically result in platelet activation. In contrast to a receptor mediated 
      activation Ca(2+)-ATPase inhibitors require the self-amplification mechanism of 
      endogenously formed thromboxane A2 to cause a similar response. We conclude that 
      the calcium store sensitive to Ca(2+)-ATPase inhibitors is a subset of the 
      receptor sensitive calcium pool.
FAU - Brune, B
AU  - Brune B
AD  - Faculty of Biology, University of Konstanz, Germany.
FAU - Ullrich, V
AU  - Ullrich V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Arachidonic Acids)
RN  - 0 (Hydroquinones)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Terpenes)
RN  - 26XK13B61B (2,5-di-tert-butylhydroquinone)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 3.4.21.5 (Thrombin)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Arachidonic Acids/metabolism
MH  - Blood Platelets/*metabolism
MH  - Calcium/*blood
MH  - Calcium-Transporting ATPases/*antagonists & inhibitors
MH  - Cytosol/metabolism
MH  - Endoplasmic Reticulum/enzymology
MH  - Humans
MH  - Hydroquinones/*pharmacology
MH  - Platelet Aggregation
MH  - Receptors, Cell Surface/metabolism
MH  - Terpenes/*pharmacology
MH  - Thapsigargin
MH  - Thrombin/pharmacology
EDAT- 1991/06/17 00:00
MHDA- 1991/06/17 00:01
CRDT- 1991/06/17 00:00
PHST- 1991/06/17 00:00 [pubmed]
PHST- 1991/06/17 00:01 [medline]
PHST- 1991/06/17 00:00 [entrez]
AID - 0014-5793(91)80747-Q [pii]
AID - 10.1016/0014-5793(91)80747-q [doi]
PST - ppublish
SO  - FEBS Lett. 1991 Jun 17;284(1):1-4. doi: 10.1016/0014-5793(91)80747-q.