PMID- 16480580
OWN - NLM
STAT- MEDLINE
DCOM- 20061020
LR  - 20150313
VI  - 25
IP  - 2
DP  - 2006 Feb
TI  - [Inhibitory effect of human brain myelin basic protein on H2O2-induced apoptosis 
      of human lung cancer cell line YTLMC-90].
PG  - 170-4
AB  - BACKGROUND & OBJECTIVE: Human brain myelin basic protein (MBP) distributes in 
      nervous system and other tissues extensively, and can be detected in many kinds 
      of tumor cells, such as lung cancer, breast cancer, and neuroglioma. However, it 
      has not been reported whether MBP is relevant to the activity of neural invasion 
      of tumors and whether MBP plays a role in biological behaviors of human lung 
      cancer cells. This study was to investigate the inhibitory effect of MBP on 
      hydrogen peroxide (H2O2)-induced apoptosis of human lung cancer cell line 
      YTLMC-90. METHODS: YTLMC-90 cells were transfected with plasmid pSVCEPMBPCAT 
      containing MBP cDNA minigene (test group), or empty vector pSVCEPCAT, or received 
      no transfection (control group), and exposed to H2O2. The expression of MBP in 
      YTLMC-90 cells was detected by Western blot. Cell proliferation was measured by 
      MTT assay. The morphologic and ultra-structural changes of apoptotic cells were 
      observed by microscopy with fluorescent staining of acridine orange (AO) and 
      electron microscopy. The DNA fragmentation was examined by agarose gel 
      electrophoresis. RESULTS: After exposed to 200 micromol/L H2O2 for 24 h, the 
      inhibitory rate of cell growth was significantly lower in test group than in 
      empty vector group and control group (36.67% vs. 78.67% and 84.00%, P<0.001). The 
      morphologic and biochemical changes of apoptotic cells, such as shrinkage of 
      cytoplasm and nucleus, fragmentation of chromatin, and ladder pattern of DNA, 
      were commonly observed in cells in control group, but these apoptotic features 
      were not discovered in test group. CONCLUSION: MBP markedly inhibits H2O2 
      cytotoxicity to YTLMC-90 cells through promoting cell proliferation and 
      antagonizing H2O2-induced apoptosis.
FAU - Chen, Jian-Ye
AU  - Chen JY
AD  - Institute of Biochemistry and Molecular Biology, West China School of Preclinical 
      and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P. R. China.
FAU - Wang, Xiao-Ming
AU  - Wang XM
FAU - Liu, Ji
AU  - Liu J
FAU - Chen, Jing-Xin
AU  - Chen JX
FAU - Wang, Ruo-Han
AU  - Wang RH
FAU - Peng, Wen-Zhen
AU  - Peng WZ
FAU - Cheng, Han-Hua
AU  - Cheng HH
FAU - Chen, Jun-Jie
AU  - Chen JJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Ai Zheng
JT  - Ai zheng = Aizheng = Chinese journal of cancer
JID - 9424852
RN  - 0 (DNA, Complementary)
RN  - 0 (Myelin Basic Protein)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - *Apoptosis/drug effects
MH  - Carcinoma, Squamous Cell/metabolism/*pathology/ultrastructure
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - DNA Fragmentation
MH  - DNA, Complementary/genetics
MH  - Humans
MH  - Hydrogen Peroxide/antagonists & inhibitors/*pharmacology
MH  - Lung Neoplasms/metabolism/*pathology/ultrastructure
MH  - Myelin Basic Protein/genetics/*metabolism
EDAT- 2006/02/17 09:00
MHDA- 2006/10/21 09:00
CRDT- 2006/02/17 09:00
PHST- 2006/02/17 09:00 [pubmed]
PHST- 2006/10/21 09:00 [medline]
PHST- 2006/02/17 09:00 [entrez]
AID - 1000467X2006020170 [pii]
PST - ppublish
SO  - Ai Zheng. 2006 Feb;25(2):170-4.