PMID- 16481070
OWN - NLM
STAT- MEDLINE
DCOM- 20060725
LR  - 20220311
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 48
IP  - 8
DP  - 2006 Jun
TI  - Depletion of intracellular Ca2+ store itself may be a major factor in 
      thapsigargin-induced ER stress and apoptosis in PC12 cells.
PG  - 696-702
AB  - The mechanisms of intracellular calcium store depletion and store-related Ca(2+) 
      dysregulation in relation to apoptotic cell death in PC12 cells were investigated 
      at physiological temperatures with a leak-resistant fluorescent indicator dye 
      Fura-PE3/AM by a cooled CCD imaging analysis system. Electron microscopic 
      observations have shown thapsigargin (TG; 100 nM)-induced apoptosis in PC12 
      cells. Thorough starvation of stored Ca(2+) by BAPTA/AM (50 microM), or La(3+) 
      (100 microM) enhanced while dantrolene (100 microM) attenuated the TG-induced 
      apoptosis by preventing a calcium release from internal stores. An immunoblotting 
      analysis revealed an enhanced expression of GRP78, the hallmark of endoplasmic 
      reticulum (ER) stress when cells were treated by TG along with BAPTA/AM. These 
      results indicate that the depletion of the intracellular Ca(2+) stores itself 
      induces the ER stress and apoptosis in PC12 cells without any involvement of the 
      capacitative calcium entry (CCE) or a sustained elevation of intracellular Ca(2+) 
      concentrations ([Ca(2+)](i)).
FAU - Yoshida, Ichiro
AU  - Yoshida I
AD  - Department of Neuropsychiatry, Graduate School of Medical Sciences, Kyushu 
      University, Higashi-ku, Fukuoka 812-8582, Japan.
FAU - Monji, Akira
AU  - Monji A
FAU - Tashiro, Ken-ichiro
AU  - Tashiro K
FAU - Nakamura, Kei-ichiro
AU  - Nakamura K
FAU - Inoue, Ryuji
AU  - Inoue R
FAU - Kanba, Shigenobu
AU  - Kanba S
LA  - eng
PT  - Journal Article
DEP - 20060214
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Chelating Agents)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (GRP78 protein, rat)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Muscle Relaxants, Central)
RN  - 0 (fura-PE3)
RN  - 67526-95-8 (Thapsigargin)
RN  - 6I3K30563S (Lanthanum)
RN  - F64QU97QCR (Dantrolene)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/*physiology
MH  - Calcium/*metabolism
MH  - Calcium Signaling/*drug effects/physiology
MH  - Chelating Agents/pharmacology
MH  - Dantrolene/pharmacology
MH  - Endoplasmic Reticulum/drug effects/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Fura-2/analogs & derivatives
MH  - Heat-Shock Proteins/drug effects/metabolism
MH  - Homeostasis/drug effects/physiology
MH  - Intracellular Fluid/drug effects/metabolism
MH  - Lanthanum/pharmacology
MH  - Molecular Chaperones/drug effects/metabolism
MH  - Muscle Relaxants, Central/pharmacology
MH  - Nerve Degeneration/metabolism/physiopathology
MH  - Neurons/drug effects/*metabolism
MH  - PC12 Cells
MH  - Rats
MH  - Stress, Physiological/chemically induced/*metabolism/physiopathology
MH  - Thapsigargin/pharmacology
EDAT- 2006/02/17 09:00
MHDA- 2006/07/26 09:00
CRDT- 2006/02/17 09:00
PHST- 2005/12/13 00:00 [received]
PHST- 2005/12/13 00:00 [accepted]
PHST- 2006/02/17 09:00 [pubmed]
PHST- 2006/07/26 09:00 [medline]
PHST- 2006/02/17 09:00 [entrez]
AID - S0197-0186(06)00018-0 [pii]
AID - 10.1016/j.neuint.2005.12.012 [doi]
PST - ppublish
SO  - Neurochem Int. 2006 Jun;48(8):696-702. doi: 10.1016/j.neuint.2005.12.012. Epub 
      2006 Feb 14.