PMID- 16481109
OWN - NLM
STAT- MEDLINE
DCOM- 20060630
LR  - 20091119
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 399
IP  - 1-2
DP  - 2006 May 15
TI  - Effects of food restriction on synthesis and expression of brain-derived 
      neurotrophic factor and tyrosine kinase B in dentate gyrus granule cells of adult 
      rats.
PG  - 135-40
AB  - We have previously found that the dendritic trees of dentate gyrus granule cells 
      are selectively vulnerable to food restriction but there are reorganizational 
      morphological events that minimize functional impairments. As the neurotrophin 
      brain-derived neurotrophic factor (BDNF) and the cognate receptor tyrosine kinase 
      B (TrkB) are involved in the maintenance of the structure of dendritic trees, we 
      thought of interest to verify if there are alterations in its synthesis and 
      expression in granule cells. To investigate this issue, 2-month-old rats were 
      submitted to 40% caloric restriction for 6 months and compared to controls fed ad 
      libitum. The numbers of granule cells containing BDNF and TrkB proteins were 
      estimated from immunostained sections and the respective mRNA levels of 
      individual neurons evaluated using nonradioactive in situ hybridization. After 
      dietary treatment there was a 15% reduction of BDNF-immunoreactive granule cells 
      with no changes of the number of TrkB-immunostained neurons. No alterations were 
      found in the levels of BDNF and TrkB mRNAs of individual granule cells. As 
      caloric restriction extends the lifespan of animals, the restrictive dietary 
      regimens are generally regarded as beneficial to the organisms, but the present 
      results suggest that caution is needed when extrapolating to some neuronal 
      populations.
FAU - Andrade, Jose P
AU  - Andrade JP
AD  - Department of Anatomy, Porto Medical School, Al. Prof. Hernani Monteiro, 4200-319 
      Porto, Portugal. jandrade@med.up.pt
FAU - Mesquita, Rui
AU  - Mesquita R
FAU - Assuncao, Marco
AU  - Assuncao M
FAU - Pereira, Pedro A
AU  - Pereira PA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060214
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Caloric Restriction
MH  - Dentate Gyrus/cytology/*metabolism
MH  - *Food Deprivation
MH  - In Situ Hybridization
MH  - Male
MH  - Neurons/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/*biosynthesis/genetics
EDAT- 2006/02/17 09:00
MHDA- 2006/07/01 09:00
CRDT- 2006/02/17 09:00
PHST- 2005/11/28 00:00 [received]
PHST- 2006/01/13 00:00 [revised]
PHST- 2006/01/24 00:00 [accepted]
PHST- 2006/02/17 09:00 [pubmed]
PHST- 2006/07/01 09:00 [medline]
PHST- 2006/02/17 09:00 [entrez]
AID - S0304-3940(06)00088-7 [pii]
AID - 10.1016/j.neulet.2006.01.043 [doi]
PST - ppublish
SO  - Neurosci Lett. 2006 May 15;399(1-2):135-40. doi: 10.1016/j.neulet.2006.01.043. 
      Epub 2006 Feb 14.