PMID- 16481319
OWN - NLM
STAT- MEDLINE
DCOM- 20060605
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 281
IP  - 15
DP  - 2006 Apr 14
TI  - Identification of the segments of the mouse transferrin receptor 1 required for 
      mouse mammary tumor virus infection.
PG  - 10243-9
AB  - Most enveloped viruses enter cells through binding of virion surface envelope 
      proteins to receptors found on the plasma membrane of the cell. The beta 
      retrovirus mouse mammary tumor virus (MMTV) uses transferrin receptor 1 (TfR1) to 
      enter cells in a pH-dependent mechanism, probably co-trafficking with TfR1 to an 
      acidic compartment where virus entry occurs. We have shown here that, although 
      mouse and rat TfR1 function as entry receptors, cat, dog, hamster, or human TfR1s 
      do not support MMTV infection. We also demonstrated that MMTV entry is 
      independent of transferrin, iron, and the TfR1 cofactor hereditary 
      hematochromatosis HFE protein. Using chimeric mouse/human hybrid TfR1 constructs, 
      we determined the site of interaction with MMTV and found that it maps to two 
      segments physically disparate from the TfR and HFE binding sites. Thus, MMTV has 
      apparently evolved to enter cells independently of the iron status of the host.
FAU - Wang, Enxiu
AU  - Wang E
AD  - Department of Microbiology and Abramson Family Cancer Center, University of 
      Pennsylvania, Rm. 313 BRB II/III, 421 Curie Boulevard, Philadelphia, PA 19104, 
      USA.
FAU - Albritton, Lorraine
AU  - Albritton L
FAU - Ross, Susan R
AU  - Ross SR
LA  - eng
GR  - R01 CA 73746/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060215
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Hemochromatosis Protein)
RN  - 0 (Hfe protein, mouse)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Membrane Proteins)
RN  - 0 (Oligopeptides)
RN  - 0 (Receptors, Transferrin)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transferrin)
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Binding, Competitive
MH  - Blotting, Western
MH  - Cell Line
MH  - Epithelial Cells/metabolism
MH  - Fibroblasts/metabolism/virology
MH  - Flow Cytometry
MH  - Hemochromatosis Protein
MH  - Histocompatibility Antigens Class I/chemistry
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Iron/chemistry
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Membrane Proteins/chemistry
MH  - Mice
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Oligopeptides/chemistry
MH  - Protein Binding
MH  - Rats
MH  - Receptors, Transferrin/chemistry/*physiology
MH  - Recombinant Fusion Proteins/chemistry/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Transferrin/chemistry
EDAT- 2006/02/17 09:00
MHDA- 2006/06/06 09:00
CRDT- 2006/02/17 09:00
PHST- 2006/02/17 09:00 [pubmed]
PHST- 2006/06/06 09:00 [medline]
PHST- 2006/02/17 09:00 [entrez]
AID - S0021-9258(19)56376-1 [pii]
AID - 10.1074/jbc.M511572200 [doi]
PST - ppublish
SO  - J Biol Chem. 2006 Apr 14;281(15):10243-9. doi: 10.1074/jbc.M511572200. Epub 2006 
      Feb 15.