PMID- 16481400
OWN - NLM
STAT- MEDLINE
DCOM- 20060808
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 17
IP  - 5
DP  - 2006 May
TI  - Casein kinase II and calcineurin modulate TRPP function and ciliary localization.
PG  - 2200-11
AB  - Cilia serve as sensory devices in a diversity of organisms and their defects 
      contribute to many human diseases. In primary cilia of kidney cells, the 
      transient receptor potential polycystin (TRPP) channels polycystin-1 (PC-1) and 
      polycystin-2 (PC-2) act as a mechanosensitive channel, with defects resulting in 
      autosomal dominant polycystic kidney disease. In sensory cilia of Caenorhabditis 
      elegans male-specific neurons, the TRPPs LOV-1 and PKD-2 are required for mating 
      behavior. The mechanisms regulating TRPP ciliary localization and function are 
      largely unknown. We identified the regulatory subunit of the serine-threonine 
      casein kinase II (CK2) as a binding partner of LOV-1 and human PC-1. CK2 and the 
      calcineurin phosphatase TAX-6 modulate male mating behavior and PKD-2 ciliary 
      localization. The phospho-defective mutant PKD-2(S534A) localizes to cilia, 
      whereas a phospho-mimetic PKD-2(S534D) mutant is largely absent from cilia. 
      Calcineurin is required for PKD-2 ciliary localization, but is not essential for 
      ciliary gene expression, ciliogenesis, or localization of cilium structural 
      components. This unanticipated function of calcineurin may be important for 
      regulating ciliary protein localization. A dynamic 
      phosphorylation-dephosphorylation cycle may represent a mechanism for modulating 
      TRPP activity, cellular sensation, and ciliary protein localization.
FAU - Hu, Jinghua
AU  - Hu J
AD  - Division of Pharmaceutical Sciences, University of Wisconsin, Madison, WI 53705, 
      USA.
FAU - Bae, Young-Kyung
AU  - Bae YK
FAU - Knobel, Karla M
AU  - Knobel KM
FAU - Barr, Maureen M
AU  - Barr MM
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060215
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (TRPP Cation Channels)
RN  - 0 (polycystic kidney disease 1 protein)
RN  - 0 (polycystic kidney disease 2 protein)
RN  - EC 2.7.11.1 (Casein Kinase II)
RN  - EC 3.1.3.- (calcineurin phosphatase)
RN  - EC 3.1.3.16 (Calcineurin)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Caenorhabditis elegans/anatomy & histology/chemistry/*metabolism
MH  - Caenorhabditis elegans Proteins/analysis/*metabolism
MH  - Calcineurin/*metabolism
MH  - Casein Kinase II/*metabolism
MH  - Cilia/chemistry/metabolism
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Phosphoric Monoester Hydrolases/metabolism
MH  - Protein Interaction Mapping
MH  - Sexual Behavior, Animal
MH  - TRPP Cation Channels/analysis/*metabolism
PMC - PMC1446073
EDAT- 2006/02/17 09:00
MHDA- 2006/08/09 09:00
PMCR- 2006/06/24
CRDT- 2006/02/17 09:00
PHST- 2006/02/17 09:00 [pubmed]
PHST- 2006/08/09 09:00 [medline]
PHST- 2006/02/17 09:00 [entrez]
PHST- 2006/06/24 00:00 [pmc-release]
AID - E05-10-0935 [pii]
AID - 2200 [pii]
AID - 10.1091/mbc.e05-10-0935 [doi]
PST - ppublish
SO  - Mol Biol Cell. 2006 May;17(5):2200-11. doi: 10.1091/mbc.e05-10-0935. Epub 2006 
      Feb 15.