PMID- 16481739
OWN - NLM
STAT- MEDLINE
DCOM- 20070123
LR  - 20200930
IS  - 1538-4047 (Print)
IS  - 1538-4047 (Linking)
VI  - 5
IP  - 4
DP  - 2006 Apr
TI  - BAM-SiPc, a novel agent for photodynamic therapy, induces apoptosis in human 
      hepatocarcinoma HepG2 cells by a direct mitochondrial action.
PG  - 413-8
AB  - Photodynamic therapy is recently developed as an effective treatment for 
      malignant disease. The therapeutic effect depends on the properties of the 
      photosensitizers. Among the novel photosensitizers we have synthesized, the 
      unsymmetrical bisamino phthalocyanine, SiPc[C3H5(NMe2)2O](OMe) (BAM-SiPc) is 
      particularly active in the HepG2 cell culture model. Fluorescence microscopy has 
      also indicated that it targets the mitochondria. In the present investigation, 
      the biochemical mechanisms of BAM-SiPc leading to cell death were investigated. 
      Photodynamic treatment with BAM-SiPc resulted in the generation of reactive 
      oxygen species and a collapse of mitochondrial membrane potential. The 
      proapoptotic Bax protein was translocated from the cytosol to mitochondria; while 
      the level of the mitochondrial anti-apoptotic Bcl-2 protein decreased after 
      photodynamic treatment. Cytochrome c, but not apoptosis-inducing factor, was 
      released from the mitochondria into the cytosol, subsequently resulting in the 
      cleavage of poly(ADP-ribose) polymerase. These events were at least partially 
      responsible for the observed BAM-SiPc induced apoptosis, which was clearly 
      demonstrated by (a) the loss of membrane asymmetry, (b) DNA ladder formation, and 
      (c) the presence of terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL)-positive cells.
FAU - Lai, Janice C
AU  - Lai JC
AD  - Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., 
      Hong Kong, China.
FAU - Lo, Pui-Chi
AU  - Lo PC
FAU - Ng, Dennis K P
AU  - Ng DK
FAU - Ko, Wing-Hung
AU  - Ko WH
FAU - Leung, Stanley C H
AU  - Leung SC
FAU - Fung, Kwok-Pui
AU  - Fung KP
FAU - Fong, Wing-Ping
AU  - Fong WP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060414
PL  - United States
TA  - Cancer Biol Ther
JT  - Cancer biology & therapy
JID - 101137842
RN  - 0 (BAM-SiPc)
RN  - 0 (Indoles)
RN  - 0 (Organosilicon Compounds)
RN  - 0 (Photosensitizing Agents)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - *Apoptosis
MH  - Carcinoma, Hepatocellular/*pathology/*therapy
MH  - Cell Line, Tumor
MH  - Cell Survival
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Indoles/*pharmacology
MH  - Membrane Potentials/drug effects
MH  - Microscopy, Fluorescence
MH  - Mitochondria/*metabolism
MH  - Organosilicon Compounds/*pharmacology
MH  - Photochemotherapy/*methods
MH  - Photosensitizing Agents/*pharmacology
MH  - Reactive Oxygen Species
EDAT- 2006/02/17 09:00
MHDA- 2007/01/24 09:00
CRDT- 2006/02/17 09:00
PHST- 2006/02/17 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/02/17 09:00 [entrez]
AID - 2513 [pii]
AID - 10.4161/cbt.5.4.2513 [doi]
PST - ppublish
SO  - Cancer Biol Ther. 2006 Apr;5(4):413-8. doi: 10.4161/cbt.5.4.2513. Epub 2006 Apr 
      14.