PMID- 16482576
OWN - NLM
STAT- MEDLINE
DCOM- 20060413
LR  - 20181201
IS  - 0736-0266 (Print)
IS  - 0736-0266 (Linking)
VI  - 24
IP  - 3
DP  - 2006 Mar
TI  - In utero transplantation of human bone marrow-derived multipotent mesenchymal 
      stem cells in mice.
PG  - 301-12
AB  - Mesenchymal stem cells (MSCs) are multipotent cells that can be isolated from 
      human bone marrow and possess the potential to differentiate into progenies of 
      embryonic mesoderm. However, current evidence is based predominantly on in vitro 
      experiments. We used a murine model of in utero transplantation (IUT) to study 
      the engraftment capabilities of human MSCs. MSCs were obtained from bone marrow 
      by negative immunoselection and limiting dilution, and were characterized by flow 
      cytometry and by in vitro differentiation into osteoblasts, chondrocytes, and 
      adipocytes. MSCs were transplanted into fetal mice at a gestational age of 14 
      days. Engraftment of human MSCs was determined by flow cytometry, polymerase 
      chain reaction, and fluorescence in situ hybridization (FISH). MSCs engrafted 
      into tissues originating from all three germ layers and persisted for up to 4 
      months or more after delivery, as evidenced by the expression of the 
      human-specific beta-2 microglobulin gene and by FISH for donor-derived cells. 
      Donor-derived CD45+ cells were detectable in the peripheral blood of recipients, 
      suggesting the participation of MSCs in hematopoiesis at the fetal stage. This 
      model can further serve to evaluate possible applications of MSCs.
CI  - Copyright 2006 Orthopaedic Research Society.
FAU - Chou, Shiu-Huey
AU  - Chou SH
AD  - Department of Life Science, Fu-Jen University, 510 Zhongzheng Road, Hsinehuang 
      City, Taipei 242, Taiwan, Republic of China. bio0004@mails.fju.edu.tw
FAU - Kuo, Tom K
AU  - Kuo TK
FAU - Liu, Ming
AU  - Liu M
FAU - Lee, Oscar K
AU  - Lee OK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Biomarkers)
RN  - 0 (beta 2-Microglobulin)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Adipocytes/cytology/metabolism
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Cell Differentiation
MH  - Chondrocytes/cytology/metabolism
MH  - Female
MH  - Fetus/*cytology
MH  - Gene Expression
MH  - Humans
MH  - Leukocyte Common Antigens/metabolism
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Models, Animal
MH  - Multipotent Stem Cells/*cytology/metabolism/transplantation
MH  - Osteoblasts/cytology/metabolism
MH  - Pregnancy
MH  - Transplantation, Heterologous
MH  - beta 2-Microglobulin/genetics/metabolism
EDAT- 2006/02/17 09:00
MHDA- 2006/04/14 09:00
CRDT- 2006/02/17 09:00
PHST- 2006/02/17 09:00 [pubmed]
PHST- 2006/04/14 09:00 [medline]
PHST- 2006/02/17 09:00 [entrez]
AID - 10.1002/jor.20047 [doi]
PST - ppublish
SO  - J Orthop Res. 2006 Mar;24(3):301-12. doi: 10.1002/jor.20047.