PMID- 16483555
OWN - NLM
STAT- MEDLINE
DCOM- 20060726
LR  - 20181113
IS  - 0006-8993 (Print)
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1077
IP  - 1
DP  - 2006 Mar 10
TI  - Neonatal 3,4-methylenedioxymethamphetamine (MDMA) exposure alters neuronal 
      protein kinase A activity, serotonin and dopamine content, and [35S]GTPgammaS 
      binding in adult rats.
PG  - 178-86
AB  - Recreational use of methylenedioxymethamphetamine (MDMA) has dramatically 
      increased among juveniles and young adults of child-bearing age, and the 
      potential for fetal exposure has increased. For this reason, it is surprising 
      that comparatively few studies have assessed the long-term impact of early MDMA 
      exposure on serotonin (5-HT) and dopamine (DA) neurotransmitter systems. The 
      purpose of this study was to determine whether repeated exposure to MDMA during 
      the preweanling period would cause long-term changes in 5-HT and DA functioning. 
      Rats were treated with saline or 20 mg/kg MDMA (two injections per day) from 
      postnatal day (PD) 11-20. At PD 90, rats were killed, and their dorsal striatum, 
      prefrontal cortex, and hippocampus were removed. 5-HT and DA content, as well as 
      their metabolites, were measured using HPLC. In addition, cAMP-dependent protein 
      kinase A (PKA) activity and agonist-stimulated [35S]GTPgammaS binding was assayed 
      using tissue homogenates from each brain region. Results indicated that early 
      MDMA exposure caused a decrease in PKA activity and 5-HT content in the 
      prefrontal cortex and hippocampus while increasing the efficacy of 5-HT1A 
      receptors as measured by agonist-stimulated [35S]GTPgammaS binding. Additionally, 
      DA content was reduced in the dorsal striatum and prefrontal cortex. These data 
      indicate that early MDMA exposure has long-term effects on the 5-HT and DA 
      neurotransmitter systems that may be mediated, at least partially, by changes in 
      5-HT1A receptor sensitivity.
FAU - Crawford, Cynthia A
AU  - Crawford CA
AD  - Department of Psychology, California State University, San Bernardino, CA 92407, 
      USA. ccrawfor@csusb.edu
FAU - Williams, Michael T
AU  - Williams MT
FAU - Kohutek, Jodie L
AU  - Kohutek JL
FAU - Choi, Fiona Y
AU  - Choi FY
FAU - Yoshida, Shelly T
AU  - Yoshida ST
FAU - McDougall, Sanders A
AU  - McDougall SA
FAU - Vorhees, Charles V
AU  - Vorhees CV
LA  - eng
GR  - K01 DA014269-05/DA/NIDA NIH HHS/United States
GR  - R01 DA021394-02/DA/NIDA NIH HHS/United States
GR  - DA006733/DA/NIDA NIH HHS/United States
GR  - K01 DA014269/DA/NIDA NIH HHS/United States
GR  - R01 DA021394/DA/NIDA NIH HHS/United States
GR  - R01 DA006733/DA/NIDA NIH HHS/United States
GR  - DA014269/DA/NIDA NIH HHS/United States
GR  - R01 DA006733-17/DA/NIDA NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060214
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Hallucinogens)
RN  - 102-32-9 (3,4-Dihydroxyphenylacetic Acid)
RN  - 333DO1RDJY (Serotonin)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - 3,4-Dihydroxyphenylacetic Acid/metabolism
MH  - Age Factors
MH  - Animals
MH  - Animals, Newborn
MH  - Brain/cytology/*drug effects/enzymology
MH  - Cyclic AMP-Dependent Protein Kinases/*drug effects
MH  - Dopamine/*metabolism
MH  - Female
MH  - Follow-Up Studies
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/*metabolism
MH  - Hallucinogens/pharmacology
MH  - Hippocampus/cytology/drug effects/enzymology
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Neurons/*drug effects/enzymology
MH  - Prosencephalon/cytology/drug effects/enzymology
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serotonin/*metabolism
MH  - Sex Factors
PMC - PMC2888305
MID - NIHMS207856
EDAT- 2006/02/18 09:00
MHDA- 2006/07/27 09:00
PMCR- 2010/06/21
CRDT- 2006/02/18 09:00
PHST- 2005/10/21 00:00 [received]
PHST- 2005/12/30 00:00 [revised]
PHST- 2006/01/06 00:00 [accepted]
PHST- 2006/02/18 09:00 [pubmed]
PHST- 2006/07/27 09:00 [medline]
PHST- 2006/02/18 09:00 [entrez]
PHST- 2010/06/21 00:00 [pmc-release]
AID - S0006-8993(06)00083-7 [pii]
AID - 10.1016/j.brainres.2006.01.017 [doi]
PST - ppublish
SO  - Brain Res. 2006 Mar 10;1077(1):178-86. doi: 10.1016/j.brainres.2006.01.017. Epub 
      2006 Feb 14.