PMID- 16484784
OWN - NLM
STAT- MEDLINE
DCOM- 20060313
LR  - 20071114
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 112
IP  - 3-4
DP  - 2006
TI  - Linkage mapping and physical localization of the major histocompatibility complex 
      region of the marsupial Monodelphis domestica.
PG  - 277-85
AB  - We used genetic linkage mapping and fluorescence in situ hybridization (FISH) to 
      conduct the first analysis of genic organization and chromosome localization of 
      the major histocompatibility complex (MHC) of a marsupial, the gray, short-tailed 
      opossum Monodelphis domestica. Family based linkage analyses of two M. domestica 
      MHC Class I genes (UA1, UG) and three MHC Class II genes (DAB, DMA, and DMB) 
      revealed that these genes were tightly linked and positioned in the central 
      region of linkage group 3 (LG3). This cluster of MHC genes was physically mapped 
      to the centromeric region of chromosome 2q by FISH using a BAC clone containing 
      the UA1 gene. An interesting finding from the linkage analyses is that 
      sex-specific recombination rates were virtually identical within the MHC region. 
      This stands in stark contrast to the genome-wide situation, wherein males exhibit 
      approximately twice as much recombination as females, and could have evolutionary 
      implications for maintaining equality between males and females in the ability to 
      generate haplotype diversity in this region. These analyses also showed that 
      three non-MHC genes that flank the MHC region on human chromosome 6, myelin 
      oligodendrocyte glycoprotein (MOG), bone morphogenetic protein 6 (BMP6), and 
      prolactin (PRL), are split among two separate linkage groups (chromosomes) in M. 
      domestica. Comparative analysis with eight other vertebrate species suggests 
      strong conservation of the BMP6-PRL synteny among birds and mammals, although the 
      BMP6-PRL-MHC-ME1 synteny is not conserved.
CI  - 2006 S. Karger AG, Basel
FAU - Gouin, N
AU  - Gouin N
AD  - Department of Genetics, Southwest Foundation for Biomedical Research, San 
      Antonio, TX 78245-0549, USA. ngouin@darwin.sfbr.org
FAU - Deakin, J E
AU  - Deakin JE
FAU - Miska, K B
AU  - Miska KB
FAU - Miller, R D
AU  - Miller RD
FAU - Kammerer, C M
AU  - Kammerer CM
FAU - Graves, J A M
AU  - Graves JA
FAU - VandeBerg, J L
AU  - VandeBerg JL
FAU - Samollow, P B
AU  - Samollow PB
LA  - eng
GR  - RR-14214/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - DNA, Complementary/genetics
MH  - Genes, MHC Class I
MH  - Genes, MHC Class II
MH  - *Major Histocompatibility Complex
MH  - Models, Genetic
MH  - Monodelphis/*genetics
MH  - Polymorphism, Genetic
MH  - Polymorphism, Restriction Fragment Length
EDAT- 2006/02/18 09:00
MHDA- 2006/03/15 09:00
CRDT- 2006/02/18 09:00
PHST- 2005/05/24 00:00 [received]
PHST- 2005/06/28 00:00 [accepted]
PHST- 2006/02/18 09:00 [pubmed]
PHST- 2006/03/15 09:00 [medline]
PHST- 2006/02/18 09:00 [entrez]
AID - 89882 [pii]
AID - 10.1159/000089882 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2006;112(3-4):277-85. doi: 10.1159/000089882.