PMID- 16487251
OWN - NLM
STAT- MEDLINE
DCOM- 20060331
LR  - 20220316
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 143
IP  - 3
DP  - 2006 Mar
TI  - Differentiation and immune function of human dendritic cells following infection 
      by respiratory syncytial virus.
PG  - 513-22
AB  - RSV causes annual epidemics of bronchiolitis in winter months resulting in the 
      hospitalization of many infants and the elderly. Dendritic cells (DCs) play a 
      pivotal role in coordinating immune responses to infection and some viruses skew, 
      or subvert, the immune functions of DCs. RSV infection of DCs could alter their 
      function and this could explain why protection after natural RSV infection is 
      incomplete and of short duration. In this study, this interaction between DCs and 
      RSV was investigated using a human primary culture model. DCs were generated from 
      purified healthy adult volunteer peripheral blood monocytes. Effects of RSV upon 
      DC phenotype with RSV primed DCs was measured using flow cytometry. Changes to 
      viability and proliferation of cocultured DCs and T-cells were determined using 
      microscopy with fluorescent dyes (Hoechst 33342 and propidium iodide). DC 
      maturation was not prevented by the RSV challenge. RSV infected a fraction of DCs 
      (10-30%) but the virus replicated slowly in these cells with only small reduction 
      to cell viability. DCs challenged with RSV stimulated T-cell proliferation less 
      well than lipopolysaccharide. This is the first study to demonstrate RSV 
      infection of human monocyte derived DCs and suggests that the virus does not 
      significantly interfere with the function of these cells and potentially may 
      promote cellular rather than humoral responses.
FAU - Jones, A
AU  - Jones A
AD  - Health and Safety Laboratory, Buxton, UK.
FAU - Morton, I
AU  - Morton I
FAU - Hobson, L
AU  - Hobson L
FAU - Evans, G S
AU  - Evans GS
FAU - Everard, M L
AU  - Everard ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
SB  - IM
MH  - Adult
MH  - Bronchiolitis, Viral/immunology
MH  - Cell Differentiation/immunology
MH  - Cell Proliferation
MH  - Cell Survival/immunology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology/pathology/virology
MH  - Flow Cytometry/methods
MH  - Humans
MH  - Immunophenotyping
MH  - Lymphocyte Activation/immunology
MH  - Respiratory Syncytial Virus Infections/*immunology
MH  - Respiratory Syncytial Viruses/isolation & purification/physiology
MH  - T-Lymphocytes/immunology
PMC - PMC1809602
EDAT- 2006/02/21 09:00
MHDA- 2006/04/01 09:00
PMCR- 2007/03/01
CRDT- 2006/02/21 09:00
PHST- 2006/02/21 09:00 [pubmed]
PHST- 2006/04/01 09:00 [medline]
PHST- 2006/02/21 09:00 [entrez]
PHST- 2007/03/01 00:00 [pmc-release]
AID - CEI3004 [pii]
AID - 10.1111/j.1365-2249.2005.03004.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2006 Mar;143(3):513-22. doi: 10.1111/j.1365-2249.2005.03004.x.