PMID- 16487486
OWN - NLM
STAT- MEDLINE
DCOM- 20060428
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 342
IP  - 2
DP  - 2006 Apr 7
TI  - Development of liver regenerative therapy using glycoside-modified bone marrow 
      cells.
PG  - 434-40
AB  - Several recent studies have reported that bone marrow cells (BMCs) have the 
      ability to generate functional hepatocytes. However, the efficiency at which BMC 
      transplantation generates functional hepatocytes is rather low. We assumed that 
      if BMCs accumulated directly in liver, the functional BMC-derived hepatocytes 
      should increase efficiently. We tried to increase the accumulation of BMCs 
      directly in liver through the interaction between hepatic asialoglycoprotein 
      receptor and desialylated BMCs. Desialylated BMCs were produced with treatment of 
      neuraminidase. Desialylated BMCs that expressed green fluorescent protein (GFP) 
      were injected into Long Evans Cinnamon (LEC) rats, a human Wilson's disease 
      model, intravenously. At 3 and 5 months after transplantation, GFP-expressing 
      hepatocyte nodules appeared in the liver of these BMC-transplanted LEC rats. 
      These findings suggest that the functional BMC-derived hepatocytes can be 
      generated by the direct accumulation of BMCs and that this strategy is new BMC 
      therapy for liver regeneration.
FAU - Misawa, Ryosuke
AU  - Misawa R
AD  - Department of Organ Regeneration, Institute of Organ Transplants, Reconstructive 
      Medicine and Tissue Engineering, Shinshu University Graduate School of Medicine, 
      3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
FAU - Ise, Hirohiko
AU  - Ise H
FAU - Takahashi, Masafumi
AU  - Takahashi M
FAU - Morimoto, Hajime
AU  - Morimoto H
FAU - Kobayashi, Eiji
AU  - Kobayashi E
FAU - Miyagawa, Shin-ichi
AU  - Miyagawa S
FAU - Ikeda, Uichi
AU  - Ikeda U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060209
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Asialoglycoprotein Receptor)
RN  - 0 (Glycosides)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.2.1.18 (Neuraminidase)
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Asialoglycoprotein Receptor/physiology
MH  - Bone Marrow Cells/*metabolism
MH  - *Bone Marrow Transplantation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Galactose/biosynthesis
MH  - Genes, Reporter
MH  - Glycosides/*metabolism
MH  - Green Fluorescent Proteins/biosynthesis/genetics
MH  - Hepatocytes/metabolism
MH  - Immunohistochemistry
MH  - Liver Diseases/*therapy
MH  - Liver Regeneration/genetics/*physiology
MH  - Male
MH  - Neuraminidase/pharmacology
MH  - Rats
MH  - Rats, Inbred LEC
MH  - Rats, Wistar
EDAT- 2006/02/21 09:00
MHDA- 2006/04/29 09:00
CRDT- 2006/02/21 09:00
PHST- 2006/01/05 00:00 [received]
PHST- 2006/01/28 00:00 [accepted]
PHST- 2006/02/21 09:00 [pubmed]
PHST- 2006/04/29 09:00 [medline]
PHST- 2006/02/21 09:00 [entrez]
AID - S0006-291X(06)00259-2 [pii]
AID - 10.1016/j.bbrc.2006.01.169 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Apr 7;342(2):434-40. doi: 
      10.1016/j.bbrc.2006.01.169. Epub 2006 Feb 9.