PMID- 16489409
OWN - NLM
STAT- MEDLINE
DCOM- 20131212
LR  - 20060220
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 58
IP  - 1
DP  - 2006 Feb 25
TI  - [Effects of tetramethylpyrazine on large-conductance Ca(2)(+)-activated potassium 
      channels in porcine coronary artery smooth muscle cells].
PG  - 83-9
AB  - The aim of the present study was to examine the effects of tetramethylpyrazine 
      (TMP) on large-conductance Ca(2+)-activated potassium channels (BK(Ca) channels) 
      in porcine coronary artery smooth muscle cells, in order to provide the 
      experimental evidence for expounding the mechanism of TMP in dilating coronary 
      artery. Cell-attached and inside-out single channel recording techniques were 
      used to observe the effects of TMP on BK(Ca) channels as well as the effects 
      after the cells were treated by protein kinase A (PKA) inhibitor or protein 
      kinase G (PKG) inhibitor. In inside-out patch, TMP activated BK(Ca) channels by 
      increasing open-state probability (N(Po)) and decreasing close time (Tc) in a 
      concentration-dependent manner. TMP (0.73~8.07 mmol/L) in the bath solution 
      increased N(Po) from (0.01+/-0.003) to (0.03+/-0.01)~(1.21+/-0.18) (P<0.01, 
      n=10), and decreased Tc from (732.33+/-90.67) ms to (359.67+/-41.30) ~ 
      (2.96+/-0.52) ms (P<0.01, n=10). These actions of TMP occurred even when the free 
      Ca(2+) concentration in the bath was reduced to ~ 0 mmol/L. The specific 
      inhibitors of PKA (H-89, 3 mumol/L) and PKG (KT-5823, 1 mumol/L) had no influence 
      on the activation of TMP on BK(Ca) channels. These findings suggest that TMP can 
      directly activate BK(Ca) channels in coronary artery smooth muscle, which 
      probably is an important mechanism in dilating coronary artery.
FAU - Yang, Yan-Yan
AU  - Yang YY
AD  - Institute of Myocardial Electrophysiology, Luzhou Medical College, Luzhou 646000, 
      China.
FAU - Yang, Yan
AU  - Yang Y
FAU - Zeng, Xiao-Rong
AU  - Zeng XR
FAU - Liu, Zhi-Fei
AU  - Liu ZF
FAU - Cai, Fang
AU  - Cai F
FAU - Li, Miao-Ling
AU  - Li ML
FAU - Zhou, Wen
AU  - Zhou W
FAU - Pei, Jie
AU  - Pei J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (Potassium Channels, Calcium-Activated)
RN  - 0 (Pyrazines)
RN  - 0 (Vasodilator Agents)
RN  - V80F4IA5XG (tetramethylpyrazine)
SB  - IM
MH  - Animals
MH  - Coronary Vessels/*cytology
MH  - Muscle, Smooth, Vascular/cytology/*metabolism
MH  - Patch-Clamp Techniques
MH  - Potassium Channels, Calcium-Activated/*drug effects/physiology
MH  - Pyrazines/*pharmacology
MH  - Swine
MH  - Vasodilator Agents/*pharmacology
EDAT- 2006/02/21 09:00
MHDA- 2013/12/18 06:00
CRDT- 2006/02/21 09:00
PHST- 2006/02/21 09:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
PHST- 2006/02/21 09:00 [entrez]
PST - ppublish
SO  - Sheng Li Xue Bao. 2006 Feb 25;58(1):83-9.