PMID- 16489415
OWN - NLM
STAT- MEDLINE
DCOM- 20070809
LR  - 20121115
IS  - 0923-9820 (Print)
IS  - 0923-9820 (Linking)
VI  - 17
IP  - 6
DP  - 2006 Dec
TI  - Biodegradation kinetics of 2,4,6-trichlorophenol by an acclimated mixed microbial 
      culture under aerobic conditions.
PG  - 535-44
AB  - The objective of this study was to achieve a better quantitative understanding of 
      the kinetics of 2,4,6-trichlorophenol (TCP) biodegradation by an acclimated mixed 
      microbial culture. An aerobic mixed microbial culture, obtained from the aeration 
      basin of the wastewater treatment plant, was acclimated in shake flasks utilizing 
      various combinations of 2,4,6-TCP (25-100 mg l(-1)), phenol (300 mg l(-1)) and 
      glycerol (2.5 mg l(-1)) as substrates. Complete primary TCP degradation and a 
      corresponding stoichiometric release of chloride ion were observed by HPLC and 
      IEC analytical techniques, respectively. The acclimated cultures were then used 
      as an inoculum for bench scale experiments in a 4 l stirred-tank reactor (STR) 
      with 2,4,6-TCP as the sole carbon/energy (C/E) source. The phenol acclimated 
      mixed microbial culture consisted of primarily Gram positive and negative rods 
      and was capable of degrading 2,4,6-TCP completely. None of the predicted 
      intermediate compounds were detected by gas chromatography in the cell cytoplasm 
      or supernatant. Based on the disappearance of 2,4,6-TCP, degradation was well 
      modelled by zero-order kinetics which was also consistent with the observed 
      oxygen consumption. Biodegradation rates were compared for four operating 
      conditions including two different initial 2,4,6-TCP concentrations and two 
      different initial biomass concentrations. While the specific rate constant was 
      not dependent on the initial 2,4,6-TCP concentration, it did depend on the 
      initial biomass concentration (X (init)). A lower biomass concentration gave a 
      much higher zero-order specific degradation rate. This behaviour was attributed 
      to a lower average biomass age or cell retention time (theta(x)) for these 
      cultures. The implications of this investigation are important for determining 
      and predicting the potential risks associated with TCP, its degradation in the 
      natural environment or the engineering implications for ex situ treatment of 
      contaminated ground water or soil.
FAU - Snyder, Christopher J P
AU  - Snyder CJ
AD  - Golder Associates Ltd., 2 180 Meadovale Boulevard, L5N 5S3, Mississauga, ON, 
      Canada.
FAU - Asghar, M
AU  - Asghar M
FAU - Scharer, Jeno M
AU  - Scharer JM
FAU - Legge, Raymond L
AU  - Legge RL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060217
PL  - Netherlands
TA  - Biodegradation
JT  - Biodegradation
JID - 9100834
RN  - 0 (Chlorophenols)
RN  - 0 (Environmental Pollutants)
RN  - MHS8C5BAUZ (2,4,6-trichlorophenol)
SB  - IM
MH  - Acclimatization
MH  - *Aerobiosis
MH  - *Biodegradation, Environmental
MH  - Biomass
MH  - Chlorophenols/*metabolism
MH  - Environmental Pollutants/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Oxygen Consumption
MH  - Water Microbiology
EDAT- 2006/02/21 09:00
MHDA- 2007/08/10 09:00
CRDT- 2006/02/21 09:00
PHST- 2005/07/11 00:00 [received]
PHST- 2005/09/27 00:00 [accepted]
PHST- 2006/02/21 09:00 [pubmed]
PHST- 2007/08/10 09:00 [medline]
PHST- 2006/02/21 09:00 [entrez]
AID - 10.1007/s10532-005-9024-8 [doi]
PST - ppublish
SO  - Biodegradation. 2006 Dec;17(6):535-44. doi: 10.1007/s10532-005-9024-8. Epub 2006 
      Feb 17.