PMID- 1649010
OWN - NLM
STAT- MEDLINE
DCOM- 19910821
LR  - 20190824
IS  - 0009-2797 (Print)
IS  - 0009-2797 (Linking)
VI  - 78
IP  - 3
DP  - 1991
TI  - Effect of enzyme inducers on metabolism of 1-nitropyrene in human hepatoma cell 
      line HepG2.
PG  - 253-68
AB  - We measured the response of HepG2 cells to the classic cytochrome (cyt.) P-450 
      inducers 3-methylcholanthrene (3-MC) and phenobarbital (PB), by evaluating 
      oxidative and/or reductive metabolism of the nitroarenes, 1-NP and 
      1,6-dinitropyrene (1,6-DNP), in control and induced cells. In HepG2 cells, 3-MC 
      induces ring-hydroxylation of 1-NP, whereas PB stimulates its nitroreduction. PB 
      induces NADPH-cyt. c reductase, but does not affect other cytosolic and 
      microsomal enzymes which contribute to 1-NP nitroreduction in these cells. 
      However, PB-inducible nitroreductase activity seems to be associated primarily 
      with cyt. P-450 isoenzymatic form(s), as indicated by the requirement for NADPH 
      and the response to specific inhibitors such as alpha-naphthoflavone and CO.
FAU - Belisario, M A
AU  - Belisario MA
AD  - Dipartimento di Biochimica e Biotecnologie Mediche, Universita degli Studi di 
      Napoli, Italy.
FAU - Arena, A R
AU  - Arena AR
FAU - Pecce, R
AU  - Pecce R
FAU - Borgia, R
AU  - Borgia R
FAU - Staiano, N
AU  - Staiano N
FAU - De Lorenzo, F
AU  - De Lorenzo F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Chem Biol Interact
JT  - Chemico-biological interactions
JID - 0227276
RN  - 0 (Mutagens)
RN  - 0 (Pyrenes)
RN  - 56-49-5 (Methylcholanthrene)
RN  - 66Q2ZUF83N (1,6-dinitropyrene)
RN  - TD1665I8Q4 (1-nitropyrene)
RN  - YQE403BP4D (Phenobarbital)
SB  - IM
MH  - Carcinoma, Hepatocellular/enzymology/*metabolism
MH  - Enzyme Induction
MH  - Humans
MH  - Liver Neoplasms/enzymology/*metabolism
MH  - Methylcholanthrene/*pharmacology
MH  - Mutagens/metabolism
MH  - Phenobarbital/*pharmacology
MH  - Pyrenes/*metabolism
MH  - Subcellular Fractions/enzymology
MH  - Tumor Cells, Cultured
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 0009-2797(91)90057-E [pii]
AID - 10.1016/0009-2797(91)90057-e [doi]
PST - ppublish
SO  - Chem Biol Interact. 1991;78(3):253-68. doi: 10.1016/0009-2797(91)90057-e.