PMID- 1649239
OWN - NLM
STAT- MEDLINE
DCOM- 19910822
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 50
IP  - 2
DP  - 1991 Aug
TI  - Effect of tumor necrosis factor on the generation of chlorinated oxidants by 
      adherent human neutrophils.
PG  - 131-9
AB  - Human neutrophils adherent to simulated biologic surfaces undergo significant 
      activation of the respiratory burst over prolonged periods of time in response to 
      stimulation with the cytokines tumor necrosis factor-alpha (TNF alpha) or tumor 
      necrosis factor-beta (TNF beta) or with the chemotactic peptide 
      N-formyl-methionylleucylphenylalanine (FMLP). In this study, neutrophils were 
      examined for their ability to generate the highly reactive and powerful oxidant 
      hypochlorous acid (HOCl) and the longer-lived, less reactive endogenous 
      nitrogen-chlorine (N-Cl) derivatives in response to these stimuli either alone or 
      when exposed to recombinant human TNF alpha (rTNF alpha) or beta (rTNF beta) 
      prior to addition of FMLP. Neutrophils adherent to fetal bovine serum-coated 
      polystyrene tissue culture wells were able to generate only small quantities of 
      HOCl when incubated with rTNF alpha, rTNF beta, or FMLP individually. However, 
      when neutrophils were first incubated with either rTNF alpha or rTNF beta prior 
      to addition of FMLP, there was a marked increase in HOCl generation. Neutrophils 
      stimulated in such a manner consumed approximately 18% of the HOCl generated in 
      the formation of N-Cl derivatives. Further scrutiny of the response to the 
      combination of rTNF alpha and FMLP revealed that HOCl release was rapid, with 80% 
      of total HOCl accumulation occurring within 15 min after FMLP addition. The 
      amount of HOCl generated was dependent on the number of cells added and on the 
      concentration of both rTNF alpha and FMLP. Comparison of HOCl generation with 
      superoxide anion and myeloperoxidase release showed that the amount of HOCl 
      generated was limited primarily by the amount of myeloperoxidase released rather 
      than by the degree of respiratory burst activation. These results demonstrate 
      that human neutrophils stimulated with FMLP after a brief incubation with rTNF 
      alpha or rTNF beta can generate cytotoxic and microbicidal concentrations of 
      chlorinated oxidants.
FAU - Test, S T
AU  - Test ST
AD  - Children's Hospital Oakland Research Institute, California 94609.
LA  - eng
GR  - DK-32094/DK/NIDDK NIH HHS/United States
GR  - HL-20985/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 11062-77-4 (Superoxides)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 712K4CDC10 (Hypochlorous Acid)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Cell Adhesion
MH  - Cell Separation/methods
MH  - Humans
MH  - Hypochlorous Acid/*blood
MH  - Kinetics
MH  - L-Lactate Dehydrogenase/blood
MH  - Lymphotoxin-alpha/*pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/drug effects/*physiology
MH  - Peroxidase/blood
MH  - Recombinant Proteins/pharmacology
MH  - Superoxides/*blood
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
AID - 10.1002/jlb.50.2.131 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1991 Aug;50(2):131-9. doi: 10.1002/jlb.50.2.131.