PMID- 1649319
OWN - NLM
STAT- MEDLINE
DCOM- 19910819
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 65
IP  - 8
DP  - 1991 Aug
TI  - Monoclonal antibodies to gp100 inhibit penetration of human herpesvirus 6 and 
      polykaryocyte formation in susceptible cells.
PG  - 4124-9
AB  - We report the derivation and properties of a monoclonal antibody (MAb 2E4) which 
      neutralizes human herpesvirus 6 (HHV-6). MAb 2E4 precipitated from lysates of 
      infected cells a glycosylated polypeptide 100,000 in apparent molecular weight 
      and minor components of 80,000, and 32,500. The predominant reactive protein 
      after a pulse was the 100,000-molecular-weight peptide designated as gp100. The 
      smaller polypeptides appeared in the precipitate predominantly after a chase. MAb 
      2E4 neutralized HHV-6 infectivity in the presence and in the absence of 
      complement, and it inhibited the penetration of virus into the cells. Addition of 
      MAb 2E4 as late as 6 h postinfection inhibited the formation of large 
      polykaryocytes typical of HHV-6-infected cells.
FAU - Foa-Tomasi, L
AU  - Foa-Tomasi L
AD  - Department of Experimental Pathology, University of Bologna, Italy.
FAU - Boscaro, A
AU  - Boscaro A
FAU - di Gaeta, S
AU  - di Gaeta S
FAU - Campadelli-Fiume, G
AU  - Campadelli-Fiume G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Proteins)
RN  - 9007-36-7 (Complement System Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/biosynthesis/*immunology
MH  - Cell Fusion
MH  - Complement System Proteins/immunology
MH  - Cytopathogenic Effect, Viral
MH  - Fluorescent Antibody Technique
MH  - Giant Cells/*immunology
MH  - Glycoproteins/chemistry/*immunology/metabolism
MH  - Herpesvirus 6, Human/*immunology/physiology
MH  - Humans
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Weight
MH  - Precipitin Tests
MH  - T-Lymphocytes/cytology/*microbiology
MH  - Viral Proteins/chemistry/*immunology/metabolism
PMC - PMC248845
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
PMCR- 1991/08/01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
PHST- 1991/08/01 00:00 [pmc-release]
AID - 10.1128/JVI.65.8.4124-4129.1991 [doi]
PST - ppublish
SO  - J Virol. 1991 Aug;65(8):4124-9. doi: 10.1128/JVI.65.8.4124-4129.1991.