PMID- 16495380
OWN - NLM
STAT- MEDLINE
DCOM- 20060928
LR  - 20161019
IS  - 1046-6673 (Print)
IS  - 1046-6673 (Linking)
VI  - 17
IP  - 4
DP  - 2006 Apr
TI  - Role of ubiquitin-like protein FAT10 in epithelial apoptosis in renal disease.
PG  - 996-1004
AB  - Dysregulated apoptosis of renal tubular epithelial cells (RTEC) is an important 
      component of the pathogenesis of several renal diseases, including HIV-associated 
      nephropathy (HIVAN), the most common cause of chronic kidney failure in 
      HIV-infected patients. In HIVAN, RTEC become infected by HIV-1 in a focal 
      distribution, and HIV-1 infection has been shown to induce apoptosis in vitro. In 
      microarray studies that used a novel renal tubular epithelial cell line from a 
      patient with HIVAN, it was found that the ubiquitin-like protein FAT10 is one of 
      the most upregulated genes in HIV-infected cells. Previously, FAT10 was shown to 
      induce apoptosis in murine fibroblasts. The expression of FAT10 in HIVAN and the 
      ability of FAT10 to induce apoptosis in human RTEC therefore were studied. This 
      study revealed that FAT10 expression is induced after infection of RTEC by HIV-1 
      and that expression of FAT10 induces apoptosis in RTEC in vitro. Moreover, it was 
      found that inhibition of endogenous FAT10 expression abrogated HIV-induced 
      apoptosis of RTEC. Immunohistochemical studies demonstrated increased FAT10 
      expression in a murine model of HIVAN, in HIVAN biopsy samples, and in autosomal 
      dominant polycystic kidney disease, another renal disease that is characterized 
      by cystic tubular enlargement and epithelial apoptosis. These results suggest a 
      novel role for FAT10 in epithelial apoptosis, which is an important component of 
      the pathogenesis of many renal diseases.
FAU - Ross, Michael J
AU  - Ross MJ
AD  - Mount Sinai School of Medicine, Division of Nephrology, Box 1243, One Gustave L. 
      Levy Place, New York, NY 10029, USA. michael.ross@mssm.edu
FAU - Wosnitzer, Matthew S
AU  - Wosnitzer MS
FAU - Ross, Michael D
AU  - Ross MD
FAU - Granelli, Benedetta
AU  - Granelli B
FAU - Gusella, G Luca
AU  - Gusella GL
FAU - Husain, Mohammad
AU  - Husain M
FAU - Kaufman, Lewis
AU  - Kaufman L
FAU - Vasievich, Matthew
AU  - Vasievich M
FAU - D'Agati, Vivette D
AU  - D'Agati VD
FAU - Wilson, Patricia D
AU  - Wilson PD
FAU - Klotman, Mary E
AU  - Klotman ME
FAU - Klotman, Paul E
AU  - Klotman PE
LA  - eng
GR  - K08 DK062672/DK/NIDDK NIH HHS/United States
GR  - K08 DK 62672/DK/NIDDK NIH HHS/United States
GR  - P01 DK 056492/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060222
PL  - United States
TA  - J Am Soc Nephrol
JT  - Journal of the American Society of Nephrology : JASN
JID - 9013836
RN  - 0 (DNA, Complementary)
RN  - 0 (FAT10 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (UBD protein, human)
RN  - 0 (Ubiquitins)
SB  - IM
MH  - AIDS-Associated Nephropathy/genetics/metabolism/pathology
MH  - Animals
MH  - Apoptosis/physiology
MH  - Base Sequence
MH  - Cell Line
MH  - DNA, Complementary/genetics
MH  - Gene Expression
MH  - HIV-1
MH  - Humans
MH  - Kidney Diseases/genetics/*metabolism/*pathology
MH  - Kidney Tubules, Proximal/metabolism/pathology
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Polycystic Kidney, Autosomal Dominant/genetics/metabolism/pathology
MH  - RNA, Messenger/genetics/metabolism
MH  - Ubiquitins/genetics/*metabolism
EDAT- 2006/02/24 09:00
MHDA- 2006/09/29 09:00
CRDT- 2006/02/24 09:00
PHST- 2006/02/24 09:00 [pubmed]
PHST- 2006/09/29 09:00 [medline]
PHST- 2006/02/24 09:00 [entrez]
AID - ASN.2005070692 [pii]
AID - 10.1681/ASN.2005070692 [doi]
PST - ppublish
SO  - J Am Soc Nephrol. 2006 Apr;17(4):996-1004. doi: 10.1681/ASN.2005070692. Epub 2006 
      Feb 22.