PMID- 16495564
OWN - NLM
STAT- MEDLINE
DCOM- 20060605
LR  - 20181113
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 74
IP  - 3
DP  - 2006 Mar
TI  - Pneumococcal polysaccharides interact with human dendritic cells.
PG  - 1890-5
AB  - Dendritic cells (DCs) are critical antigen presentation cells whose influence on 
      murine immune responses to polysaccharide antigens has only recently been 
      elucidated. Little is known about human DC-polysaccharide interactions. We set 
      out to study the interaction between human monocyte-derived DCs and pneumococcal 
      capsular polysaccharides (PPS) in vitro. Immature DCs were generated from 
      peripheral blood monocytes and incubated with fluorescein isothiocyanate-labeled 
      PPS type 9N or 14 for assessment of uptake. DCs were exposed to PPS type 1, 6B, 
      9N, 14, 19F, or 23F in the absence or presence of Escherichia coli 
      lipopolysaccharide (LPS) for assessment of phenotypic DC maturation and cytokine 
      production. PPS were taken up by immature DCs and proceeded to HLA-DR+ and 
      lysosome-associated membrane protein-1+ late endosomal compartments. Uptake was 
      reduced in the presence of cytochalasin D and wortmannin, suggesting that both 
      cytoskeletal rearrangements and phosphatidylinositol 3-kinase activation may be 
      required for internalization. None of the PPS tested induced DC phenotype 
      changes, maturation, or interleukin-12 (IL-12)/IL-10 production. However, PPS 
      were capable of modulating the response of the DCs to a second signal such as 
      LPS. Exposure of DCs to PPS in the presence of LPS resulted in an altered 
      cytokine balance with significantly increased IL-10 production and reduced IL-12 
      production compared to LPS alone. This effect was not seen using the control 
      antigen tetanus toxoid. DC-pneumococcus interaction may affect subsequent immune 
      responses to pneumococci, as an altered cytokine balance may have a profound 
      effect on DC-driven T-cell priming.
FAU - Meltzer, Ulrike
AU  - Meltzer U
AD  - Immunobiology Unit, The Institute of Child Health, Great Ormond Street Hospital, 
      University College London, 30 Guilford Street, London WC1N 1EH, United Kingdom.
FAU - Goldblatt, David
AU  - Goldblatt D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Dendritic Cells/immunology/*metabolism
MH  - Humans
MH  - Lipopolysaccharides/*metabolism
MH  - Monocytes/*immunology
MH  - Pneumococcal Infections/immunology/*pathology
MH  - Streptococcus pneumoniae/*chemistry
PMC - PMC1418647
EDAT- 2006/02/24 09:00
MHDA- 2006/06/06 09:00
PMCR- 2006/07/01
CRDT- 2006/02/24 09:00
PHST- 2006/02/24 09:00 [pubmed]
PHST- 2006/06/06 09:00 [medline]
PHST- 2006/02/24 09:00 [entrez]
PHST- 2006/07/01 00:00 [pmc-release]
AID - 74/3/1890 [pii]
AID - 1439-05 [pii]
AID - 10.1128/IAI.74.3.1890-1895.2006 [doi]
PST - ppublish
SO  - Infect Immun. 2006 Mar;74(3):1890-5. doi: 10.1128/IAI.74.3.1890-1895.2006.