PMID- 1649698
OWN - NLM
STAT- MEDLINE
DCOM- 19910828
LR  - 20041117
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 51
IP  - 15
DP  - 1991 Aug 1
TI  - Elevated levels of transforming growth factor beta messenger RNA and its 
      polypeptide in human hepatocellular carcinoma.
PG  - 4080-3
AB  - Malignant cells in culture express elevated levels of transforming growth factor 
      beta 1 (TGF-beta 1) mRNA and secrete an abundant amount of TGF-beta protein, but 
      little is known about the production of TGF-beta in human malignant tissues in 
      vivo. We estimated the levels of TGF-beta 1 mRNA expression by Northern 
      hybridization and measured TGF-beta protein using a radioreceptor assay in tumor 
      tissues surgically obtained from six patients with hepatocellular carcinoma 
      (HCC). TGF-beta 1 mRNA was expressed at much higher levels in HCC tissues from 
      all the cases compared with normal human liver, suggesting an association of the 
      activated TGF-beta 1 gene transcription with hepatocarcinogenesis. The content of 
      TGF-beta was 207 +/- 121 ng/g wet tissue in the HCC tissue, and it showed 
      correlation with the level of TGF-beta 1 mRNA in the tissue (r = 0.69; P less 
      than 0.05). An immunohistochemical study demonstrated that TGF-beta 1 staining 
      could be observed in HCC cells. These observations suggest that human HCC 
      strongly expresses TGF-beta 1 mRNA in vivo, leading to a high content of TGF-beta 
      protein.
FAU - Ito, N
AU  - Ito N
AD  - Second Department of Internal Medicine, Osaka University Medical School, Japan.
FAU - Kawata, S
AU  - Kawata S
FAU - Tamura, S
AU  - Tamura S
FAU - Takaishi, K
AU  - Takaishi K
FAU - Shirai, Y
AU  - Shirai Y
FAU - Kiso, S
AU  - Kiso S
FAU - Yabuuchi, I
AU  - Yabuuchi I
FAU - Matsuda, Y
AU  - Matsuda Y
FAU - Nishioka, M
AU  - Nishioka M
FAU - Tarui, S
AU  - Tarui S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Aged
MH  - Blotting, Northern
MH  - Carcinoma, Hepatocellular/genetics/*metabolism
MH  - Female
MH  - Humans
MH  - Liver Neoplasms/genetics/*metabolism
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/genetics/*metabolism
MH  - Radioligand Assay
MH  - Transforming Growth Factor beta/biosynthesis/*genetics/metabolism
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1991 Aug 1;51(15):4080-3.