PMID- 1649703
OWN - NLM
STAT- MEDLINE
DCOM- 19910827
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 66
IP  - 2
DP  - 1991 Jul 26
TI  - TrkB mediates BDNF/NT-3-dependent survival and proliferation in fibroblasts 
      lacking the low affinity NGF receptor.
PG  - 405-13
AB  - The neurotrophins (nerve growth factor [NGF], brain-derived neurotrophic factor 
      [BDNF], neurotrophin-3 [NT-3], and neurotrophin-4 [NT-4] ) have been defined by 
      their ability to support neuronal survival. These factors utilize the Trk family 
      of receptor tyrosine kinases, perhaps in conjunction with a second component 
      known as the low affinity NGF receptor (LNGFR). Here we demonstrate that TrkB 
      mediates survival and proliferation in response to both BDNF and NT-3 when 
      expressed in a particular strain of NIH 3T3 fibroblasts, with BDNF the more 
      potent ligand. Furthermore, the BDNF dose dependency displayed by these 
      TrkB-expressing fibroblasts is similar to that required to support the survival 
      of primary neurons. The LNGFR is not expressed in our fibroblast system, implying 
      that this receptor is not essential for responses to physiological concentrations 
      of the neurotrophins. We discuss our findings in the context of neurotrophin 
      signaling pathways and mechanisms of neuronal cell death.
FAU - Glass, D J
AU  - Glass DJ
AD  - Regeneron Pharmaceuticals, Inc., Tarrytown, New York 10591.
FAU - Nye, S H
AU  - Nye SH
FAU - Hantzopoulos, P
AU  - Hantzopoulos P
FAU - Macchi, M J
AU  - Macchi MJ
FAU - Squinto, S P
AU  - Squinto SP
FAU - Goldfarb, M
AU  - Goldfarb M
FAU - Yancopoulos, G D
AU  - Yancopoulos GD
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.10.1 (Ntrk2 protein, mouse)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - DNA Replication/drug effects
MH  - Fibroblasts/cytology/drug effects
MH  - Membrane Glycoproteins/*metabolism
MH  - Mice
MH  - Nerve Growth Factors/*pharmacology
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neurotrophin 3
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Receptor, trkB
MH  - Receptors, Cell Surface/physiology
MH  - Receptors, Nerve Growth Factor
MH  - Recombinant Proteins/pharmacology
MH  - Transfection
EDAT- 1991/07/26 00:00
MHDA- 1991/07/26 00:01
CRDT- 1991/07/26 00:00
PHST- 1991/07/26 00:00 [pubmed]
PHST- 1991/07/26 00:01 [medline]
PHST- 1991/07/26 00:00 [entrez]
AID - 0092-8674(91)90629-D [pii]
AID - 10.1016/0092-8674(91)90629-d [doi]
PST - ppublish
SO  - Cell. 1991 Jul 26;66(2):405-13. doi: 10.1016/0092-8674(91)90629-d.