PMID- 16497437
OWN - NLM
STAT- MEDLINE
DCOM- 20060726
LR  - 20071115
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 399
IP  - 3
DP  - 2006 May 22
TI  - Altered expression of pro-inflammatory and developmental genes in the fetal brain 
      in a mouse model of maternal infection.
PG  - 220-5
AB  - Human studies of unexplained cerebral palsy (CP) suggest an association with 
      maternal infection. We used an established model of maternal infection, 
      lipopolysaccharide (LPS) administration, to investigate the molecular changes in 
      the fetal brain that may link maternal infection and CP. We compared gene 
      expression in brains from mouse pups exposed to LPS in utero to those from 
      saline-treated controls. Dams were injected with 50 microg LPS or saline on E18 
      with surgical delivery from 0.5 to 6h later. Differential gene expression was 
      analyzed in the whole mouse brain using RT-PCR. When compared to control mice, 
      pups exposed to LPS showed increased expression of pro-inflammatory genes 
      monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6), and 
      interleukin-1beta (IL-1beta), as well as VEGF, a regulator of vascular 
      development and permeability, the anti-apoptotic protein Y-box-binding protein-1 
      (YB-1), and the neuronal differentiation factor necdin. LPS-exposed mice also 
      showed downregulation of semaphorin 5b and groucho, involved in axon guidance and 
      neurogenesis, respectively, providing evidence that LPS may disrupt normal 
      developmental pathways. These data suggest possible mechanisms for adverse 
      neurological outcomes following maternal infection involving elevated cytokine 
      levels and altered expression of developmental genes in the fetal brain.
FAU - Liverman, Christopher S
AU  - Liverman CS
AD  - Department of Anatomy and Cell Biology, University of Kansas Medical Center, Mail 
      Stop 3038, 3901 Rainbow Blvd., Kansas City, KS 66160, USA.
FAU - Kaftan, Harold A
AU  - Kaftan HA
FAU - Cui, Lisa
AU  - Cui L
FAU - Hersperger, Stephen G
AU  - Hersperger SG
FAU - Taboada, Eugenio
AU  - Taboada E
FAU - Klein, Robert M
AU  - Klein RM
FAU - Berman, Nancy E J
AU  - Berman NE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060221
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Brain/*drug effects/embryology
MH  - Cytokines/classification/*metabolism
MH  - Disease Models, Animal
MH  - Embryo, Mammalian
MH  - Female
MH  - Gene Expression/*drug effects
MH  - Genes, Developmental/*drug effects
MH  - Lipopolysaccharides
MH  - Mice
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Time Factors
EDAT- 2006/02/25 09:00
MHDA- 2006/07/27 09:00
CRDT- 2006/02/25 09:00
PHST- 2005/11/19 00:00 [received]
PHST- 2006/01/17 00:00 [revised]
PHST- 2006/01/31 00:00 [accepted]
PHST- 2006/02/25 09:00 [pubmed]
PHST- 2006/07/27 09:00 [medline]
PHST- 2006/02/25 09:00 [entrez]
AID - S0304-3940(06)00114-5 [pii]
AID - 10.1016/j.neulet.2006.01.064 [doi]
PST - ppublish
SO  - Neurosci Lett. 2006 May 22;399(3):220-5. doi: 10.1016/j.neulet.2006.01.064. Epub 
      2006 Feb 21.